Vip3Aa induces apoptosis in cultured Spodoptera frugiperda (Sf9) cells
Vip3Aa induces apoptosis in cultured Spodoptera frugiperda (Sf9) cells
复制标题
Vip3Aa 诱导培养的草地贪夜蛾 (Sf9) 细胞凋亡
DOI：
10.1016/j.toxicon.2016.07.019
复制
发表时间：
2016-09-15
期刊：
影响因子：
2.8
通讯作者：
Cai, Jun
中科院分区：
文献类型：
--
作者：
Jiang, Kun;Mei, Si-qi;Cai, Jun
许多苏云金芽孢杆菌菌株在营养生长阶段分泌的营养杀虫蛋白（Vip）被认为是第二代杀虫蛋白，因为它们与已知的晶体杀虫蛋白（Cry）没有序列或结构同源性，并且具有广泛的杀虫谱。与杀虫晶体蛋白（ICP）相比，Vips 的杀虫机制研究较少。在这里，我们研究了培养昆虫细胞中 Vip3Aa 毒性的机制。使用流式细胞术分析、TUNEL 染色和 DNA 片段化分析，我们表明 Vip3Aa 可以诱导草地贪夜蛾 (Sf9) 细胞凋亡并导致细胞停滞在 G2/M 期。我们还表明，Vip3Aa 可以破坏线粒体膜电位 (Delta Psi m)，导致 Sf-caspase-1 激活，表明线粒体介导的 caspase 依赖性途径可能参与 Vip3Aa 诱导的 Sf9 细胞凋亡。 (C) 2016 Elsevier Ltd. 保留所有权利。
The vegetative insecticidal proteins (Vip) secreted by many Bacillus thuringiensis strains during their vegetative growth stage are regarded as second generation insecticidal proteins, as they share no sequence or structural homology with known crystal insecticidal proteins (Cry) and have a broad insecticidal spectrum. Compared with insecticidal crystal proteins (ICPs), the insecticidal mechanisms of Vips have been little studied. Here we investigated the mechanism responsible for Vip3Aa toxicity in cultured insect cells. Using, flow cytometry analyzes, TUNEL staining and DNA fragmentatioh assays, we show that Vip3Aa can induce apoptosis in Spodoptera frugiperda (Sf9) cells and cause cells to arrest at the G2/M phase. We also show that Vip3Aa can disrupt mitochondrial membrane potential (Delta Psi m), leading to the activation of Sf-caspase-1, suggesting that a mitochondrial mediated and caspase dependent pathway may be involved in Vip3Aa-induced apoptosis in Sf9 cells. (C) 2016 Elsevier Ltd. All rights reserved.