Use of electrochemical impedance measurements to monitor β-adrenergic stimulation of bovine aortic endothelial cells
Use of electrochemical impedance measurements to monitor β-adrenergic stimulation of bovine aortic endothelial cells
复制标题
使用电化学阻抗测量监测牛主动脉内皮细胞的 β-肾上腺素能刺激
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
H. Franke
中科院分区：
文献类型：
--
作者：
J. Wegener;S. Zink;P. Rösen;H. Franke
摘要 由于大血管来源的内皮细胞层的高通透性，精确地确定其对离子移动的屏障功能需要精细的实验技术。因此，我们直接在金膜电极上培养牛主动脉内皮细胞，并测量其电化学阻抗，以研究其一般和β-肾上腺素能刺激时的被动电学特性。汇流电池单层的阻抗谱(10~(-2)·106赫兹)表明，电池的电学特性可用简单的阻容并联网络来模拟。在对照条件下，BAEC单分子膜的总电阻为3.6±0.6Ω·cm2(n=30)，电容为0.6±0.1µF/cm2。关于这些细胞的形态特征，这两个量都进行了讨论。用合成的β受体激动剂异丙肾上腺素刺激BAECs后，细胞层电阻呈浓度依赖性、高度特异性增加，其半数最大反应浓度(EC_(50))为0.3±0.1微米，但细胞层电容未受影响。使用单频阻抗测量，我们分析了BAECs对异丙肾上腺素处理的反应，并与几种已知刺激3‘，5’-环磷酸腺苷(CAMP)依赖的信号转导级联的化学无关化合物进行了比较。这些研究证实，β-肾上腺素能刺激后细胞层阻力的增强是通过细胞内cAMP的增加来实现的。
Abstract Due to the high permeability of endothelial cell layers derived from macrovascular vessels, precise determination of their barrier function towards ion movement requires refined experimental techniques. We thus cultured bovine aortic endothelial cells (BAEC) directly on thin gold-film electrodes and measured the electrochemical impedance to study their passive electrical properties in general and during β-adrenergic stimulation. Impedance spectra (10–2·106 Hz) of confluent cell monolayers revealed that the electrical characteristics of the cells can be modelled by a simple resistor-capacitor parallel network. Under control conditions the overall resistance of confluent BAEC monolayers was 3.6±0.6 Ω·cm2 (n=30) and the capacitance was 0.6±0.1 µF/cm2. Both quantities are discussed with respect to morphological characteristics of these cells. Stimulation of BAECs with the synthetic β-adrenoceptor agonist isoproterenol leads to a concentration-dependent, highly specific increase of the cell layer resistance characterized by a concentration for half-maximal response (EC50) of 0.3±0.1 µM. The cell layer capacitance, however, remained unaffected. Using impedance measurements at a single frequency, we analysed the response of BAECs to treatment with isoproterenol in comparison with several chemically unrelated compounds known to stimulate the adenosine 3’,5’-cyclic monophosphate (cAMP)-dependent signal transduction cascade. These studies confirmed that the enhancement of the cell layer resistance after β-adrenergic stimulation is mediated by an increase in intracellular cAMP.
内皮细胞表面的功能。
影响因子：
18.2
作者：
Simionescu,M;Simionescu,N
通讯作者：
Simionescu,N
DOI：
10.1073/pnas.89.17.7919
发表时间：
1992-09-01
影响因子：
11.1
作者：
TIRUPPATHI, C;MALIK, AB;GIAEVER, I
通讯作者：
GIAEVER, I