A STANDARDIZED EXPERIMENTAL FRACTURE IN THE MOUSE TIBIA
A STANDARDIZED EXPERIMENTAL FRACTURE IN THE MOUSE TIBIA
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DOI：
10.1002/jor.1100110219
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发表时间：
1993-03-01
影响因子：
2.8
通讯作者：
ARO, HT
中科院分区：
文献类型：
--
作者：
HILTUNEN, A;VUORIO, E;ARO, HT
越来越多地使用转基因小鼠作为实验动物，为研究骨折修复的生物学提供了新的机会。我们已经开发出一种技术，用于生产一个标准的闭合实验骨折的小鼠胫骨。将一根0.2 mm不锈钢棒插入髓腔，并通过冲击器械使预钉胫骨干断裂，这导致了可重现的横向或轻微倾斜骨折模式。髓内棒保持轴向对齐，骨折愈合无移位。骨痂几何形状的测量，四点弯曲试验，生化分析和定量组织学的基础上，骨痂形成和重塑的进展发生在一个可预测的序列愈合阶段。骨折的极限弯曲载荷随时间增加，在4周内达到完整对照胫骨强度的74%。骨折的刚度值恢复到正常水平，并且如X线片所确定的，骨折在4周内通过外部骨痂愈合。放射学检查显示，骨痂大小、横截面积和骨痂质量在2周时达到峰值，此后下降，表明外部重塑开始。组织学上，间充质组织的量在第5天和第7天最大。骨痂软骨面积在第9天达到峰值，在其最大值，它占总骨痂面积的46%。早期膜状新骨的骨膜形成，随后是软骨内骨化，导致愈合过程中骨痂骨的线性增加。小鼠胫骨骨折的愈合顺序与大鼠胫骨中观察到的相似。主要的区别是小鼠的小尺寸，这使得手术技术和麻醉程序更加苛刻。
The increased use of transgenic mice as experimental animals provides new opportunities to study the biology of fracture repair. We have developed a technique for the production of a standard closed experimental fracture in the mouse tibia. A 0.2 mm stainless-steel rod was introduced into the medullary cavity and the pre-nailed tibial shaft was fractured by an impact device, which resulted in a reproducible transverse or slightly oblique fracture pattern. The intramedullary rod maintained axial alignment, and the fractures united without displacement. On the basis of measurements of callus geometry, four-point bending tests, biochemical analyses, and quantitative histology, the progress of callus formation and remodeling occurred in a predictable sequence of healing phases. The ultimate bending loads of the fractures increased with time, reaching 74% of the strength of intact control tibias in 4 weeks. The stiffness values of the fractures returned to normal levels and, as determined radiographically, the fractures united by external callus in 4 weeks. Radiographically, callus size, cross-sectional callus area, and callus mass peaked at 2 weeks and decreased thereafter, indicating the start of external remodeling. Histologically, the amount of mesenchymal tissue was maximal at days 5 and 7. The callus cartilage area peaked at day 9; at its maximum, it accounted for 46% of the total callus area. Early periosteal formation of membranous new bone, followed by endochondral ossification, resulted in a linear increase of callus bone during the healing process. The healing sequence of the mouse tibial fracture was similar to that seen in the rat tibia. The major difference is the small size of the mouse, which makes the surgical technique and anesthetic procedures more demanding.