Genetic analysis for rice grain quality traits in the YVB stable variant line using RAD-seq
Genetic analysis for rice grain quality traits in the YVB stable variant line using RAD-seq
复制标题
使用 RAD-seq 对 YVB 稳定变体系中的稻米品质性状进行遗传分析
DOI：
10.1007/s00438-015-1104-9
复制
发表时间：
2016-02-01
影响因子：
3.1
通讯作者：
Zhao, Bingran
中科院分区：
文献类型：
--
作者：
Peng, Yan;Hu, Yuanyi;Zhao, Bingran
水稻育种的未来可能建立在对优良品种和远缘亚种间遗传资源的进一步利用的基础上。以往的研究证明，外源基因组DNA转化方法可以将远缘亲缘（供体）的遗传信息转移到栽培稻（受体）中。然而，这种形式的遗传转移的机制的特点是很差的，导致这些变异的表型变化的基因通常很难识别。本研究以籼稻品种V20 B为材料，通过“穗茎注射法"（SIM）导入水稻基因组DNA，获得了一个稳定的、米质性状显著改善的变异系YVB。利用限制性内切酶位点相关DNA测序技术（RAD-seq）对BC 1F 5回交群体（YVB × V20 B）进行分析，构建高密度SNPs的遗传连锁图谱，用于在全基因组水平上探讨基因型-表型关系。共定位了17个稻米品质性状的数量性状基因座（QTL），分别位于第3、5、6、8和9染色体上。控制不同表型变异的8个主效QTL定位在第5染色体的同一区域。该区域含有控制粒重的GS 5基因和控制粒宽的qSW 5/GW 5基因。本研究为通过将基因组DNA导入远缘作物近缘物种来研究谷物性状表型变异和遗传信息传递的分子机制提供了新的资源和见解。
The future of rice breeding will likely be built on the basis of the further utilization of heterosis between elite cultivars and genetic resources from distant subspecies of rice. Previous studies have proved that exogenous genomic DNA transformation methods can be used to transfer genetic information from distant relatives (donor) into cultivated rice (recipient). However, the mechanism underlying this form of genetic transfer is poorly characterized, and the genes that cause the phenotypic changes in these variants are typically difficult to identify. This study examined YVB, a stable variant line with greatly improved grain quality traits that was derived from an indica variety (V20B) by transferring genomic DNA ofO.minutathrough the “spike-stalk injection method (SIM)”. We used restriction-site associated DNA sequencing technology (RAD-seq) to evaluate a population of BC1F5backcross lines (YVB × V20B); the RAD-seq data were used to construct a genetic linkage map with high-density SNPs for use in association analysis exploring genotype–phenotype relationships at the whole-genome level. A total of 17 quantitative trait loci (QTLs) for rice quality traits were mapped to chromosomes 3, 5, 6, 8, and 9. 8 major QTLs controlling different phenotypic variations were mapped to the same region of chromosome 5. This region contained theGS5gene for grain weight and theqSW5/GW5gene for grain width. This study provides new resources and insights into the molecular mechanisms of grain trait phenotypic variation and the transmission of genetic information via the introduction of genomic DNA to a distantly related crop relative species.