Heat-stress induced inhibition in growth and chlorosis in mungbean (Phaseolus aureus Roxb.) is partly mitigated by ascorbic acid application and is related to reduction in oxidative stress
Heat-stress induced inhibition in growth and chlorosis in mungbean (Phaseolus aureus Roxb.) is partly mitigated by ascorbic acid application and is related to reduction in oxidative stress
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DOI：
10.1007/s11738-011-0748-2
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发表时间：
2011-11-01
影响因子：
2.6
通讯作者：
Nayyar, Harsh
中科院分区：
文献类型：
--
作者：
Kumar, Sanjeev;Kaur, Ramanpreet;Nayyar, Harsh
气温上升（> 35摄氏度）对夏播绿豆基因型不利，这些基因型的营养生长和生殖生长受到抑制。在本研究中，绿豆植株在30/20 A ℃（对照）、35/25、40/30和45/35 A ℃的不同温度下水培生长（白天/晚上12小时/12小时）与（50 μ M）或无抗坏血酸（ASC）的影响进行了研究，生长，膜损伤，叶绿素损失，叶片水分状况，氧化胁迫的成分，和抗氧化剂。ASC处理的植物在发芽和幼苗生长方面表现出显著的改善，特别是在40/30和45/35摄氏度下。细胞膜的损伤，水分的损失，细胞呼吸的减少，叶绿素显着防止ASC处理植物生长在这些温度下。氧化应激测量丙二醛和过氧化氢的含量，观察到显着降低在高温下与ASC应用。超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶和谷胱甘肽还原酶的活性在40/30 A ℃时升高，但在45/35 A ℃时降低，而施用ASC后，这些酶的活性明显恢复。在所有的抗氧化剂中，内源ASC含量在45/35 A ℃生长的植株中下降的程度最大，表明它在影响绿豆对热胁迫的反应中起着重要作用。在45/35 ℃时，外源ASC使内源谷胱甘肽和脯氨酸含量沿着增加。研究结果表明，热胁迫导致的生长抑制和黄化与叶片水分状况的下降和氧化胁迫的加剧有关，外源ASC可以部分地防止热胁迫引起的生长抑制和黄化。它的作用，赋予保护免受热应力进行了讨论。
The rising temperatures (> 35A degrees C) are proving detrimental to summer-sown mungbean genotypes that experience inhibition of vegetative and reproductive growth. In the present study, the mungbean plants growing hydroponically at varying temperatures of 30/20A degrees C (control), 35/25, 40/30, and 45/35A degrees C (as day/night 12 h/12 h) with (50 mu M) or without ascorbic acid (ASC) were investigated for effects on growth, membrane damage, chlorophyll loss, leaf water status, components of oxidative stress, and antioxidants. The ASC-treated plants showed significant improvement in germination and seedling growth especially at 40/30 and 45/35A degrees C. The damage to membranes, loss of water, decrease in cellular respiration, and chlorophyll were significantly prevented by ASC treatment to plants growing at these temperatures. The oxidative stress measured as malondialdehyde and hydrogen peroxide content was observed to be significantly lower at high temperatures with ASC application. The activities of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase increased at 40/30A degrees C but decreased at 45/35A degrees C in the absence of ASC while with its application, the activities of these enzymes were appreciably resorted. Among all the antioxidants, the endogenous ASC content decreased to the greatest extent at 45/35A degrees C grown plants indicating its vital role in affecting the response of mungbean to heat stress. Exogenously applied ASC raised its endogenous content along with that of glutathione and proline at 45/35A degrees C. The findings indicated that heat stress-induced inhibition in growth and chlorosis was associated with decrease in leaf water status and elevation of oxidative stress, which could partly be prevented by exogenous application of ASC. Its role in imparting protection against heat stress is discussed.