Acoustic characterisation of pH dependant reversible micellar casein aggregation
Acoustic characterisation of pH dependant reversible micellar casein aggregation
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DOI：
10.1016/j.colsurfa.2019.02.026
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发表时间：
2019-05
期刊：
影响因子：
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通讯作者：
M. J. Francis;Z. Glover;Q. Yu;M. Povey;M. Holmes
中科院分区：
文献类型：
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作者：
M. J. Francis;Z. Glover;Q. Yu;M. Povey;M. Holmes
使用超声波和基于光的测量来监测搅拌和静止溶液特性的变化，例如酪蛋白在模型胶体体系中聚集和重新分布过程中的颗粒尺寸分布。通过调节溶液的pH值来改变胶束的稳定性，从而控制酪蛋白胶束的沉淀。所研究的分散体是在高pH下制备的，通过等电点沉淀，然后以特定的间隔进一步降低pH来重新分散。在每个特定的pH区间用Zeta电位法测定等电点。酪蛋白分散体的等电点在pH 4.3~4.7范围内。成功地用声学方法测量了这种可逆酪蛋白体系的颗粒大小变化。随后，使用声学和光学法相结合的方法，在较宽的pH范围内监测溶出速度和溶液动力学。
Ultrasound and light-based measurements were used to monitor changes in agitated and quiescent solution characteristics such as particle size distribution during casein aggregation and redistribution in a model colloidal system. The precipitation of casein micelles was controlled by adjusting the pH of the solution to alter the stability of the micelles. The studied dispersions were prepared at high pH, taken through the isoelectric point to precipitate and then re-dispersed by further lowering the pH at specific intervals. The isoelectric point was determined by zeta potential measurement at each specific pH interval. The isoelectric point of the casein dispersions was in the region of pH 4.3–4.7. Changes in the particle size of this reversible casein system were successfully measured acoustically. The dissolution rate and solution kinetics were subsequently monitored in a wide pH range using a combination of acoustic and light-based methods.