Application of 2D IR Bioimaging: Hyperspectral Images of Formalin-Fixed Pancreatic Tissues and Observation of Slow Protein Degradation.
Application of 2D IR Bioimaging: Hyperspectral Images of Formalin-Fixed Pancreatic Tissues and Observation of Slow Protein Degradation.
复制标题
DOI：
10.1021/acs.jpcb.1c05554
复制
发表时间：
2021-08-26
期刊：
影响因子：
--
通讯作者：
Zanni MT
中科院分区：
文献类型：
--
作者：
Dicke SS;Alperstein AM;Schueler KL;Stapleton DS;Simonett SP;Fields CR;Chalyavi F;Keller MP;Attie AD;Zanni MT
我们使用二维红外生物成像研究福尔马林固定的小鼠胰腺的结构异质性。通过绘制与酰胺I振动模式相关的量，从高光谱数据集生成图像，酰胺I振动模式是由主链羰基拉伸产生的。图像测量的基本振动频率，交叉峰，和非谐位移。为每个量生成直方图，提供平均值和平均值周围的分布，作为蛋白质结构的度量。图像是从储存在福尔马林固定3、8和48周的组织中生成的。在此期间，所有三个指标均显示样品的β-折叠含量增加，与蛋白质聚集一致。我们的研究结果表明，福尔马林固定并不能完全阻止胰腺组织中蛋白质结构的降解。
We used two-dimensional IR bioimaging to study the structural heterogeneity of formalin-fixed mouse pancreas. Images were generated from the hyperspectral data sets by plotting quantities associated with the amide I vibrational mode, which is created by the backbone carbonyl stretch. Images that measure the fundamental vibrational frequencies, cross peaks, and anharmonic shifts are presented. Histograms are generated for each quantity, providing averaged values and distributions around the mean that serve as metrics for protein structures. Images were generated from tissue that had been stored in a formalin fixation for 3, 8, and 48 weeks. Over this period, all three metrics show that that the β-sheet content of the samples increased, consistent with protein aggregation. Our results indicate that formalin fixation does not entirely arrest the degradation of a protein structure in pancreas tissue.
登录
查看更多内容
DOI：
10.1016/j.bbapap.2010.04.001
发表时间：
2010-07
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Biancalana M;Koide S
通讯作者：
Koide S
影响因子：
3.8
作者：
Chen, Zhixing;Wei, Lu;Min, Wei
通讯作者：
Min, Wei
影响因子：
3.5
作者：
Bhargava R
通讯作者：
Bhargava R
DOI：
10.1021/acs.jpcb.5b07706
发表时间：
2015-11-05
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Dunkelberger EB;Grechko M;Zanni MT
通讯作者：
Zanni MT
影响因子：
5
作者：
Howie, Alexander J.;Brewer, Douglas B.;Jones, Adrian P.
通讯作者：
Jones, Adrian P.