Differential expression and regulation of Runx1 in mouse uterus during the peri-implantation period
Differential expression and regulation of Runx1 in mouse uterus during the peri-implantation period
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Runx1在小鼠围着床期子宫中的差异表达及调控
DOI：
10.1007/s00441-015-2174-z
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发表时间：
2015-10-01
影响因子：
3.6
通讯作者：
Yue, Zhan-Peng
中科院分区：
文献类型：
--
作者：
Bai, Zhi-Kun;Li, Dang-Dang;Yue, Zhan-Peng
Runx 1转录因子是一个重要的发育调节因子。然而，很少有人知道Runx 1对胚胎着床和蜕膜化的影响。本研究的目的是研究Runx 1在围着床期小鼠子宫中的表达和调控。妊娠第1-4天未见明显Runx 1 mRNA信号。在妊娠第5天，Runx 1 mRNA主要定位于植入囊胚周围的腔下基质中。在雌激素激活的着床子宫中观察到类似的结果。同时，Runx 1 mRNA在妊娠第6-8天和人工蜕膜化条件下表达水平较高。8-Br-cAMP可诱导子宫间质细胞Runx 1 mRNA的表达。PKA抑制剂H89可明显阻断该诱导作用。用特异性siRNA抑制Runx 1可以降低子宫基质细胞的增殖和蜕膜标志物Prl 8a 2和Prl 3c 1在子宫基质细胞中的表达。进一步研究发现，抑制Runx 1还可抑制子宫间质细胞中考克斯-2、mPGES-1和Mmp 2基因的表达。雌、孕激素可诱导去卵巢小鼠子宫及子宫基质细胞Runx 1 mRNA的表达。综上所述，这些数据表明Runx 1可能在小鼠蜕膜化过程中发挥重要作用。
Runx1 transcription factor is a key developmental regulator. However, little is known about the effects of Runx1 on embryo implantation and decidualization. The aim of this study is to examine the expression and regulation of Runx1 in mouse uterus during the peri-implantation period. There was no evident Runx1 mRNA signal on days 1-4 of pregnancy. On day 5 of pregnancy, Runx1 mRNA was mainly localized in the subluminal stroma surrounding the implanting blastocyst. A similar result was observed in the estrogen-activated implantation uterus. Simultaneously, a high level of Runx1 mRNA expression was detected on days 6-8 of pregnancy and under artificial decidualization. 8-Br-cAMP could induce the expression of Runx1 mRNA in the uterine stromal cells. Moreover, the induction was obviously blocked by PKA inhibitor H89. Inhibition of Runx1 with specific siRNA could decrease the proliferation of stromal cells and expression of decidual markers Prl8a2 and Prl3c1 in the uterine stromal cells. Further study found that inhibition of Runx1 could also suppress the expression of Cox-2, mPGES-1 and Mmp2 genes in uterine stromal cells. Estrogen and progesterone could induce the expression of Runx1 mRNA in ovariectomized mouse uterus and uterine stromal cells. Taken together, these data suggest that Runx1 may play an important role during mouse decidualization.