EFFECTS OF SONICATION AND CENTRIFUGATION OF CLINICAL SPECIMENS ON THE RECOVERY OF HERPES-SIMPLEX VIRUS
EFFECTS OF SONICATION AND CENTRIFUGATION OF CLINICAL SPECIMENS ON THE RECOVERY OF HERPES-SIMPLEX VIRUS
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DOI：
10.1016/0732-8893(88)90015-6
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发表时间：
1988-11-01
影响因子：
2.9
通讯作者：
DILWORTH, V
中科院分区：
文献类型：
--
作者：
ARENS, M;SWIERKOSZ, E;DILWORTH, V
我们已经分析了单纯疱疹病毒（HSV）的冷冻和新鲜标本，以确定超声处理和离心对病毒回收的影响。超声处理后，24/27份标本的滴度增加了1.3-30.8倍，平均增加6.8倍。在超声处理前后将174份新鲜标本接种到CV-1试管培养物中。超声处理和未超声处理部分之间的阳性时间无统计学显著差异。因此，尽管超声处理标本可以急剧增加阳性标本的病毒滴度，但超声处理新鲜标本不会显著增强HSV的分离。为了确定临床标本离心对HSV回收的影响，对31份培养阳性冷冻标本进行离心，并对上清液和重悬的超声处理沉淀进行滴定。25份标本具有足够的可回收病毒以进行滴度测定;在21/25份（87.5%）标本中，离心后上清液滴度降低（平均降低38%;范围为4-92%）。两个31（7%）上清液是阴性的培养，而超声处理的沉淀是阳性的。因此，在细胞接种前离心标本可能会影响HSV的回收。
We have analyzed frozen and fresh specimens for herpes simplex virus (HSV) to determine the effect of sonication and centrifugation on virus recovery. After sonication, titers of 24/27 specimens increased from 1.3-30.8-fold with a mean increase of 6.8-fold. One hundred seventy-four fresh specimens were inoculated before and after sonication into CV-1 tube cultures. There was not a statistically significant difference in time to positivity between the sonicated and unsonicated portions. Thus, although sonication of specimens can sharply increase the viral titer of positive specimens, sonication of fresh specimens does not significantly enhance the isolation of HSV. To determine the effect of centrifugation of clinical specimens on recovery of HSV, thirty-one culture positive frozen specimens were centrifuged, and the supernatants and resuspended sonicated pellets were titered. Twenty-five specimens had sufficient recoverable virus to titer; in 21/25 (87.5%) specimens the titers were reduced in the supernatants after centrifugation (mean reduction 38%; range of 4-92%). Two of 31 (7%) supernatants were negative in culture while the sonicated pellets were positive. Thus, centrifugation of specimens prior to cell inoculation may compromise recovery of HSV.