New Fluorogenic Substrates for α-Thrombin, Factor Xa, Kallikreins, and Urokinase
New Fluorogenic Substrates for α-Thrombin, Factor Xa, Kallikreins, and Urokinase
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用于 α-凝血酶、Xa 因子、激肽释放酶和尿激酶的新型荧光底物
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
S. Sakakibara
中科院分区：
文献类型：
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作者：
T. Morita;H. Kato;S. Iwanaga;K. Takada;Terutoshi Kimura;S. Sakakibara
新合成了20种肽-4-甲基香豆素酰胺（MCA），并对其作为α -凝血酶、Xa因子、钾溶酶、尿激酶和纤溶酶可能的底物进行了测试。这些荧光肽的羧基端含有精氨酸- mca。荧光法测定7-氨基-4-甲基香豆素的释放量。在这些肽中，发现以下是单个酶的特异性底物：boc - var - pro - arg - mca用于α凝血酶，Boc-Ile-Glu-Gly-Arg-MCA和Boc-Ser-Gly-Arg-MCA用于Xa因子，z - ph - arg - mca用于血浆钾化酶，pro - ph - arg - mca用于胰腺和尿液钾化酶，戊二酰- gly - arg - mca用于尿激酶。此外，这些肽- mca底物对纤溶酶具有抗性。
: Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides contained arginine-MCA as the carboxyl-terminus. Release of 7-amino-4-methylcoumarin was determined fluorometrically. Of these peptides, the following were found to be specific substrates for individual enzymes: Boc-Val-Pro-Arg-MCA for alpha-thrombin, Boc-Ile-Glu-Gly-Arg-MCA, and Boc-Ser-Gly-Arg-MCA for factor Xa, Z-Phe-Arg-MCA for plasma kallikrein, Pro-Phe-Arg-MCA for pancreatic and urinary kallikreins, and glutaryl-Gly-Arg-MCA for urokinase. Moreover, these peptide-MCA substrates were resistant to plasmin.