Specificity of monoclonal antibodies in local passive immunization against Streptococcus mutans.
Specificity of monoclonal antibodies in local passive immunization against Streptococcus mutans.
复制标题
单克隆抗体在局部被动免疫中针对变形链球菌的特异性。
DOI：
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发表时间：
1989
影响因子：
4.6
通讯作者：
T. Lehner
中科院分区：
文献类型：
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作者：
J. Ma;M. Hunjan;R. Smith;T. Lehner
用抗沙门氏菌185-kD抗原I/II的单克隆抗体（MoAb）对人体进行局部口服被动免疫。变异体显著减少或阻止了生物体的外源菌株的口服定殖。然而，在用生理盐水或不相关的单克隆抗体假免疫的受试者中，S。变形链球菌持续时间较长的时间比那些免疫特异性抗链球菌单克隆抗体。原住民S.在这种微生物被抗微生物剂降低到检测不到的水平后，变形杆菌也被特异性MoAb完全阻止。事实上，S。与对照受试者在10 - 82天内的变异相比，在超过1年的时间内未检测到变异。用四种单克隆抗体研究了单克隆抗体在预防链球菌定植中的特异性。这表明：（1）抗体的亚类不是必需因素，因为MoAb Guy's 1和13都能防止定植，尽管Guy's 1是IgG2a，Guy's 13是IgG1类抗体;（2）血清型特异性很重要，因为MoAb Guy's 9仅识别S。sobrinus（血清型d和g）不能阻止S.变形菌（血清型c）;（3）推测的粘附素的蛋白质或碳水化合物性质都不是决定因素，因为单抗Guy's 1识别碳水化合物，而Guy's 13识别蛋白质决定簇，这两种单抗都阻止了S的粘附。表位特异性似乎是阻止S.由于MoAb Guy's 11和13具有相同的血清型特异性，并且都识别蛋白质决定簇，但只有Guy's 13阻止定殖。长期的保护，从再殖民的土著S。在停止施用特异性MoAb后持续约1年的变异不能由保留在牙齿上的功能性MoAb来解释。我们认为，最初的单克隆抗体防止殖民的S。变形链球菌，并且这种链球菌腾出的生态位被口腔植物群中的其他生物体填充，从而阻止了变形链球菌的重新定殖。变异人
Local oral passive immunization in human subjects with a monoclonal antibody (MoAb) raised against the 185-kD antigen I/II from S. mutans significantly reduced or prevented oral colonization of an exogenous strain of the organism. In subjects sham-immunized with either saline or an unrelated MoAb, however, significantly greater proportions of S. mutans persisted for a longer duration than in those immunized with the specific anti-streptococcal MoAb. Recolonization of indigenous S. mutans after this organism was reduced to undetectable levels by an antimicrobial agent has also been completely prevented with specific MoAb. Indeed, S. mutans was not detected for a period of over 1 year, as compared with recolonization within 10-82 days in the control subjects. The specificity of MoAb in preventing colonization of the streptococci was studied with four MoAb. This revealed that: (1) the sub-class of antibody is not an essential factor, as both MoAb Guy's 1 and 13 prevented colonization, although Guy's 1 is an IgG2a and Guy's 13 is an IgG1 class of antibody; (2) serotype specificity is important, as MoAb Guy's 9, which only recognizes S. sobrinus (serotypes d and g), does not prevent colonisation by S. mutans (serotype c); (3) neither protein nor carbohydrate nature of the putative adhesin was a determining factor, because MoAb Guy's 1 recognizes a carbohydrate and Guy's 13 a protein determinant and both MoAb prevented adherence of S. mutans; and (4) epitope specificity appears to be the most important factor in preventing adherence of S. mutans, as MoAb Guy's 11 and 13 share the same serotype specificity and both recognize a protein determinant, yet only Guy's 13 prevents colonisation. The long duration of protection from re-colonization by indigenous S. mutans, lasting about 1 year after application of the specific MoAb was stopped, cannot be accounted for by functional MoAb remaining on the teeth. We suggest that initially the MoAb prevents colonization by S. mutans and that the ecological niche vacated by this streptococcus is filled by other organisms from the oral flora, thereby discouraging re-colonization by S. mutans.