Enhanced expression levels of IL-31 correlate with IL-4 and IL-13 in atopic and allergic contact dermatitis.
Enhanced expression levels of IL-31 correlate with IL-4 and IL-13 in atopic and allergic contact dermatitis.
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DOI：
10.1016/j.jaci.2006.07.015
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发表时间：
2006-10-01
影响因子：
14.2
通讯作者：
Hermanns, Heike M.
中科院分区：
文献类型：
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作者：
Neis, Mark M.;Peters, Bettina;Hermanns, Heike M.
背景：IL-31由活化的T淋巴细胞产生，优先由T（H）2细胞产生。过表达IL-31的转基因小鼠具有类似人类受试者过敏性皮炎的表型。目的：我们试图评估IL-31在人类T细胞介导的皮肤病发病机制中的潜在重要性。我们分析了从特应性皮炎（AD）、变应性接触性皮炎（ACD）用实时荧光定量PCR检测T（H）1/T（H）2细胞因子的表达，并与13例健康皮肤患者的标本进行比较。我们发现，从AD患者的活检标本中IL-31的mRNA水平在统计学上增加，无论疾病的严重程度和血清IgE水平。此外，在许多取自ACD患者的活检标本中，IL-31 mRNA水平显著升高。然而，在取自银屑病斑块的活检标本中未检测到IL-31转录的增加。IL-31与T（H）1或T（H）2细胞因子的mRNA水平的比较表明IL-31的表达与IL-4和IL-13相关，但与IFN-γ无关。IL-31受体mRNA没有显着增加，可以检测到在任何疾病，而IL-31的第二个受体亚单位，抑瘤素M受体，似乎是增强转录在patients with psoriasis.Conclusion：IL-31的表达不仅增加AD患者，但也在那些与ACD，2皮炎皮肤疾病。在两种类型的湿疹中，IL-31的表达与T（H）2细胞因子IL-4和IL-13的表达相关。临床意义：IL-31可能不仅参与AD的发展，而且参与ACD引起的皮肤炎症。
Background: IL-31 is produced by activated T lymphocytes, preferentially by T(H)2 cells. Transgenic mice overexpressing IL-31 have a phenotype resembling allergic dermatitis in human subjects.Objective: We sought to evaluate the potential importance of IL-31 in the pathogenesis of human T cell-mediated skin diseases.Methods: We analyzed total RNA taken from 149 skin biopsy specimens from patients with atopic dermatitis (AD), allergic contact dermatitis (ACD), or psoriasis in comparison with specimens taken from patients with healthy skin (n=13) by using quantitative real-time PCR for the expression of T(H)1/T(H)2 cytokines.Results: We found statistically increased mRNA levels of IL-31 in biopsy specimens taken from patients with AD, irrespective of the severity of the disease and serum IgE levels. Moreover, IL-31 mRNA levels were strongly increased in many biopsy specimens taken from patients with ACD. However, no increased transcription of IL-31 could be detected in biopsy specimens taken from psoriatic plaques. A comparison of mRNA levels of IL-31 with T(H)1 or T(H)2 cytokines demonstrates a correlation of the expression of IL-31 with IL-4 and IL-13 but not with IFN-gamma. No significant increase of IL-31 receptor mRNA could be detected in any disease, whereas the second receptor subunit of IL-31, the oncostatin M receptor, seems to be enhanced transcribed in patients with psoriasis.Conclusion: IL-31 expression is not only increased in patients with AD but also in those with ACD, 2 pruritic skin disorders. In both types of eczema, expression of IL-31 is associated with the expression of the T(H)2 cytokines IL-4 and IL-13.Clinical implications: IL-31 might contribute not only to the development of AD but also to ACD-provoked skin inflammation.