Polysaccharides of St. John'sWort Herb Stimulate NHDF Proliferation and NEHK Differentiation via Influence on Extracellular Structures and Signal Pathways
Polysaccharides of St. John'sWort Herb Stimulate NHDF Proliferation and NEHK Differentiation via Influence on Extracellular Structures and Signal Pathways
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DOI：
10.1155/2012/304317
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Deters, A. M.
中科院分区：
文献类型：
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作者：
Abakuks, S.;Deters, A. M.
圣约翰草提取物通常含有不需要的或自愿的多糖。由于多糖具有结构依赖性的生物学功能，本研究从中草药中提取水溶性多糖，通过阴离子交换层析将其分成四个主要多糖组分（命名为Hp 1、Hp 2、Hp 3和Hp 4），并通过HPAEC-PAD、CE、IR和GC-MS进行表征。通过研究它们对增殖的影响来评估对人皮肤角质形成细胞和成纤维细胞的生物活性，代谢、细胞毒性、凋亡和分化。在基因表达研究中研究了潜在的机制。多糖组分Hp 1主要由β-D-葡萄糖组成。Hp 2、Hp 3和Hp 4含有果胶结构和阿拉伯半乳聚糖蛋白，其组成和数量各不相同。Hp 1多糖通过抑制表皮生长因子和胰岛素受体基因表达诱导角质形成细胞分化。而成纤维细胞的胶原蛋白分泌刺激的每一个多糖馏分只有Hp 1刺激合成。成纤维细胞增殖被Hp 1抑制，被Hp 4促进。这种效应与影响氧化应激、代谢、转录过程和胞外蛋白等基因有关。总之，多糖已被证明是圣约翰草水提物的生物活性成分，其结构特征和功能之间的关系。
St. John's Wort herb extracts often contain undesirable or volitional polysaccharides. As polysaccharides exhibit structuredependent biological functions in the present study water-soluble polysaccharides were extracted from herb material, fractionated by anion exchange chromatography into four main polysaccharide fractions (denominated as Hp1, Hp2, Hp3 and Hp4) and characterized by HPAEC-PAD, CE, IR and GC-MS. Biological activity on human skin keratinocytes and fibroblasts was assessed by investigation of their effect on proliferation, metabolism, cytotoxicity, apoptosis and differentiation. The underlying mechanisms were investigated in gene expression studies. Polysaccharide fraction Hp1 was mainly composed of beta-D-glucose. Hp2, Hp3 and Hp4 contained pectic structures and arabinogalactan proteins varying in composition and quantity. Polysaccharides of Hp1 induced the keratinocyte differentiation by inhibiting the gene expression of the epidermal growth factor and insulin receptor. While the collagen secretion of fibroblasts was stimulated by each polysaccharide fraction only Hp1 stimulated the synthesis. The fibroblast proliferation was reduced by Hp1 and increased by Hp4. This effect was related to the influence on genes that referred to oxidative stress, metabolism, transcription processes and extracellular proteins. In conclusion polysaccharides have been shown as biologically active ingredients of aqueous St. John's Wort extracts with a relation between their structural characteristics and function.