A colorimetric and ratiometric fluorescent probe for mercury (II) in lysosome
A colorimetric and ratiometric fluorescent probe for mercury (II) in lysosome
复制标题
溶酶体中汞 (II) 的比色和比率荧光探针
DOI：
10.1016/j.snb.2015.11.018
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Jun Yin
中科院分区：
文献类型：
--
作者：
Yufeng Zhang;Haiyan Chen;Dan Chen;Di Wu;Zhao Chen;Jing Zhang;Xiaoqiang Chen;Sheng Hua Liu;Jun Yin
汞是一种高度危险且分布广泛的重金属，可导致许多严重的健康问题，如肾衰竭、中枢神经系统损害以及各种认知和运动障碍，甚至死亡。有效的汞离子检测方法具有重要意义。本工作利用光诱导电子转移机制，研制了一种基于4-氯-7-硝基苯并-2，1，3-恶二唑(NBD-Cl)的比色比值型荧光探针。此外，我们幸运地获得了与Hg2+结合前后的单晶结构，表明氨基和吗啉基团上的两个氮原子参与了配位并形成了2：2的络合物。该探针对水溶液中的汞(II)离子表现出高度的选择性响应，并具有显著的溶酶体靶向检测活细胞中Hg2+的能力。结果表明，Hg2+选择性探针具有良好的水溶性、生物相容性和细胞穿透性，可作为探测与Hg2+有关的细胞毒理作用的有效工具。
Mercury is a highly hazardous and widespread heavy metal and can cause many severe health problems such as kidney failure, central nervous system damage with various cognitive and motor disorders, and even death. Efficient approach for the detection of mercury ion is very significant. In this work, a colorimetric and ratiometric fluorescent probe based on 4-chloro-7-nitrobenzo-2,1,3-oxadiazole (NBD-Cl) is developed by employing a photo-induced electron transfer mechanism. Furthermore, we were fortunate enough to obtain the single crystal structures before and after binding with Hg2+, which displays that two nitrogen atoms on amino and morpholine groups participate in the coordination and form a complex with a ratio of 2:2. And this probe displays a highly selective response to mercury (II) ions in an aqueous solution and possesses a distinguished capability for lysosome-targeting detection of Hg2+without interferences in living cells. The results suggest that Hg2+-selective probe has good water-solubility, biocompatibility and cell-penetrability and can serve as an efficient tool for probing cellular toxicological actions that are related to Hg2+.