Electrically induced bonding of DNA to gold
Electrically induced bonding of DNA to gold
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DOI：
10.1038/nchem.722
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发表时间：
2010-09-01
期刊：
影响因子：
21.8
通讯作者：
Gaub, Hermann E.
中科院分区：
文献类型：
--
作者：
Erdmann, Matthias;David, Ralf;Gaub, Hermann E.
单分子技术的发展为超分子结构和生物分子网络的观察和组装提供了许多新方法。我们之前报道过一种称为单分子剪切粘贴方法的方法，用于拾取单个 DNA 链并将其沉积在表面上。然而，这需要用与要拾取然后沉积的DNA链互补的DNA链对表面进行预功能化。在这里，我们展示了结合到原子力显微镜尖端的双链 DNA 单分子，可以使用电触发器（表面电位循环）沉积在裸金电极上。研究了 DNA 和电极之间的相互作用，我们发现双链 DNA 仅在其末端通过伯胺基团化学吸附到金电极上。我们在实验中证实了这一发现，其中聚乙二醇间隔物上只有一个腺苷核苷酸被“电吸附”到金电极上。
The development of single-molecule techniques has afforded many new methods for the observation and assembly of supramolecular structures and biomolecular networks. We previously reported a method, known as the single-molecule cut-and-paste approach, to pick up and deposit individual DNA strands on a surface. This, however, required pre-functionalization of the surface with DNA strands complementary to those that were to be picked up and then deposited. Here we show that single molecules of double-stranded DNA, bound to the tip of an atomic force microscope, can be deposited on a bare gold electrode using an electrical trigger (surface potential cycling). The interactions between the DNA and the electrode were investigated and we found that double-stranded DNA chemisorbs to the gold electrode exclusively at its end through primary amine groups. We corroborated this finding in experiments in which only a single adenosine nucleotide on a polyethylene glycol spacer was 'electrosorbed' to the gold electrode.