Quantification of amikacin and kanamycin in serum using a simple and validated LC-MS/MS method
Quantification of amikacin and kanamycin in serum using a simple and validated LC-MS/MS method
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DOI：
10.4155/bio.14.191
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发表时间：
2014-01-01
期刊：
影响因子：
1.8
通讯作者：
Alffenaar, Jan-Willem C.
中科院分区：
文献类型：
--
作者：
Dijkstra, Jacob A.;Sturkenboom, Marieke G. G.;Alffenaar, Jan-Willem C.
背景：阿米卡星和卡那霉素常用于治疗耐多药结核病。目前市售的免疫分析法不能分析卡那霉素和阿米卡星的低谷水平。因此，目的是建立一种LC-MS/MS方法定量人血清中阿米卡星和卡那霉素。材料与方法：以阿帕霉素为内标，对选择性、准确度、精密度、回收率、基质效应和稳定性进行评价。结果：所建立的LC-MS/MS方法符合美国FDA的建议，阿米卡星的低定量限为250 ng/ml，卡那霉素的低定量限为100 ng/ml。LC-MS/MS法与阿米卡星免疫分析法（Architect(R)）比较差异无统计学意义（p = 0.501）。结论：LC-MS/MS法具有阿米卡星低定量限和对卡那霉素分析能力强的特点，是分析这些氨基糖苷的首选方法。
Background: Amikacin and kanamycin are frequently used in the treatment of multidrug-resistant TB. The current commercially available immunoassay is unable to analyze kanamycin and trough levels of amikacin. The objective was therefore to develop a LC-MS/MS method for the quantification of amikacin and kanamycin in human serum. Materials & methods: Using apramycin as internal standard, selectivity, accuracy, precision, recovery, matrix effects and stability were evaluated. Results: The presented LC-MS/MS method meets the recommendations of the US FDA with a low LLOQ of 250 ng/ml for amikacin and 100 ng/ml for kanamycin. No statistical significant difference was found between the LC-MS/MS method and the immunoassay of amikacin (Architect((R)) assay, p = 0.501). Conclusion: The low LLOQ of amikacin and the ability to analyze kanamycin makes the LC-MS/MS method the preferred method for analyzing these aminoglycosides.