Stable, specific, and reversible base pairing via Schiff base
Stable, specific, and reversible base pairing via Schiff base
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DOI：
10.1021/ja054618q
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发表时间：
2005-11-30
影响因子：
15
通讯作者：
Saito, I
中科院分区：
文献类型：
--
作者：
Dohno, C;Okamoto, A;Saito, I
我们在此提出了一种新型共价连接碱基对，通过 5-甲酰尿嘧啶 (U-f) 和 5-氨基胞嘧啶 (C-Am) 之间形成席夫碱。席夫碱键的形成可逆地进行，并且不需要任何添加剂。交联的DNA在变性条件下非常稳定，但在90℃加热时完全解离。C-Am和U-f的配对能力非常特异，适用于fU的检测，fU是DNA中T的主要氧化损伤。我们提出希夫碱基连接作为一种新的人工碱基配对方案来创建功能性 DNA。
We here present a novel covalently linked base pair via Schiff base formation between 5-formyluracil (U-f) and 5-aminocytosine (C-Am). Formation of the Schiff base linkage proceeds reversibly and does not require any additives. The cross-linked DNA is very stable under denaturing conditions, whereas it completely dissociates upon heating at 90 degrees C. The pairing ability of C-Am and U-f is very specific and is applicable to the detection of fU, which is the major oxidative lesion of T in DNA. We propose the Schiff base linkage as a new artificial base pairing scheme to create functional DNAs.