DIRECT EXPRESSION CLONING OF VASCULAR CELL-ADHESION MOLECULE-1, A CYTOKINE-INDUCED ENDOTHELIAL PROTEIN THAT BINDS TO LYMPHOCYTES
DIRECT EXPRESSION CLONING OF VASCULAR CELL-ADHESION MOLECULE-1, A CYTOKINE-INDUCED ENDOTHELIAL PROTEIN THAT BINDS TO LYMPHOCYTES
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DOI：
10.1016/0092-8674(89)90775-7
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发表时间：
1989-12-22
期刊：
影响因子：
64.5
通讯作者：
LOBB, R
中科院分区：
文献类型：
--
作者：
OSBORN, L;HESSION, C;LOBB, R
我们已经克隆了一个以前未描述的粘附分子，VCAM-1，这是诱导的细胞因子对人类内皮细胞和淋巴细胞结合。使用一种新的方法，既不需要单克隆抗体，也不需要纯化的蛋白质，VCAM-1表达克隆的选择粘附到人淋巴细胞系。VCAM-1 mRNA在用IL-1或TNF-α处理后2小时存在于内皮细胞中。并维持至少72小时;白细胞结合活性与mRNA诱导平行。用VCAM-1转染的细胞结合人白血病细胞系Jurkat、拉莫斯、Raji、HL 60和THP 1，但不结合外周血嗜中性粒细胞。VCAM-1属于免疫球蛋白基因超家族，可能是募集单核白细胞进入体内炎症部位的核心。
We have cloned a previously undescribed adhesion molecule, VCAM-1, which is induced by cytokines on human endothelial cells and binds lymphocytes. Using a novel method requiring neither monoclonal antibodies nor purified protein, VCAM-1-expressing clones were selected by adhesion to human lymphoid cell lines. VCAM-1 mRNA is present in endothelial cells at 2 hr after treatment with IL-1 or TNF-.alpha. and is maintained for at least 72 hr; leukocyte binding activity parallels mRNA induction. Cells transfected with VCAM-1 bind the human leukemia lines Jurkat, Ramos, Raji, HL60, and THP1, but not peripheral blood neutrophils. VCAM-1, which belongs to the immunoglobulin gene super-family, may be central to recruitment of mononuclear leukocytes into inflammatory sites in vivo.