Sources of edaphic cyanobacterial diversity in the Dry Valleys of Eastern Antarctica
Sources of edaphic cyanobacterial diversity in the Dry Valleys of Eastern Antarctica
复制标题
南极洲东部干谷土壤蓝藻多样性的来源
DOI：
10.1038/ismej.2007.104
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
S. Cary
中科院分区：
文献类型：
--
作者：
Susanna A. Wood;Susanna A. Wood;Andreas Rueckert;Don A. Cowan;S. Cary
蓝藻是南极干谷生态系统的主要组成部分。它们在湖泊和池塘中的出现已有充分记录，但对其在土壤环境中的分布知之甚少。关于主要源自蓝藻的水生有机物对陆地生态系统的贡献存在相当多的争论。在这项研究中，使用自动 rRNA 基因间隔区分析 (ARISA) 和 16S rRNA 基因克隆文库来研究米尔斯和灯塔谷内一系列土壤环境中的蓝藻多样性。这些数据用于阐明水生蓝藻对土壤群落的输入。从各种土壤环境中收集了 38 个样品，包括干燥和潮湿的土壤、下岩体以及湖泊和水生微生物垫。 ARISA 和 16S rRNA 克隆文库分析结果表明，米尔斯山谷的矿质土壤内存在多种蓝藻群落。灯塔谷的土壤样本中没有蓝藻信号。在米尔斯谷内，水生蓝藻垫中发现的 ARISA 片段长度的很大一部分 (29%–58%) 也存在于土壤和下石样品中，表明湖泊和水生蓝藻在构建土壤群落中发挥着重要作用。使用 BEST 分析评估非生物变量对土壤样品群落结构的影响。对米尔斯谷内样品的 BEST 分析结果表明，碳含量的总百分比是解释蓝藻群落结构差异的最重要变量。 BEST 分析表明，四种元素对山谷之间的物种组成差异有显着影响。我们认为，灯塔谷完全没有湖泊或池塘是导致这些土壤蓝藻成分低的一个因素。
Cyanobacteria are major components of Antarctic Dry Valley ecosystems. Their occurrence in lakes and ponds is well documented, however, less is known about their distribution in edaphic environments. There has been considerable debate about the contribution of aquatic organic matter derived largely from cyanobacteria to terrestrial ecosystems. In this study, automated rRNA intergenic spacer analysis (ARISA) and 16S rRNA gene clone libraries were used to investigate cyanobacterial diversity in a range of soil environments within the Miers and Beacon Valleys. These data were used to elucidate the input of aquatic cyanobacteria to soil communities. Thirty-eight samples were collected from a variety of soil environments including dry and moist soils, hypoliths and lake and hydroterrestrial microbial mats. The results from the ARISA and 16S rRNA clone library analysis demonstrated that diverse cyanobacterial communities exist within the mineral soils of the Miers Valley. The soil samples from Beacon Valley were depauparate in cyanobacterial signals. Within Miers Valley, significant portions (29%–58%) of ARISA fragment lengths found in aquatic cyanobacterial mats were also present in soil and hypolith samples, indicating that lacustrine and hydroterrestrial cyanobacteria play a significant role in structuring soil communities. The influence of abiotic variables on the community structure of soil samples was assessed using BEST analysis. The results of BEST analysis of samples from within Miers Valley showed that total percentage of carbon content was the most important variable in explaining differences in cyanobacterial community structure. The BEST analyses indicated that four elements contributed significantly to species compositional differences between valleys. We suggest that the complete absence of lakes or ponds from Beacon Valley is a contributing factor to the low cyanobacterial component of these soils.
DOI：
10.1073/pnas.82.20.6955
发表时间：
1985-01-01
影响因子：
11.1
作者：
LANE, DJ;PACE, B;PACE, NR
通讯作者：
PACE, NR