Genetic Diversity and Geographic Differentiation in the Threatened Species Dysosma pleiantha in China as Revealed by ISSR Analysis
Genetic Diversity and Geographic Differentiation in the Threatened Species Dysosma pleiantha in China as Revealed by ISSR Analysis
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DOI：
10.1007/s10528-007-9141-7
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发表时间：
2008-01
影响因子：
2.4
通讯作者：
Min Zong;Hai-long Liu;Y. Qiu;Shu-zhen Yang;Ming-shui Zhao;C. Fu
中科院分区：
文献类型：
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作者：
Min Zong;Hai-long Liu;Y. Qiu;Shu-zhen Yang;Ming-shui Zhao;C. Fu
八角莲是一种重要的濒危药用植物，仅分布于我国东南部。该物种能够进行有性和无性繁殖。在这项研究中，间简单重复序列标记数据，获得和分析的遗传变异和遗传结构。克隆性的程度，连同克隆和有性繁殖策略，不同的网站，和在恶劣的生态条件下的种群往往有较大的克隆与相对较低的克隆多样性造成的营养繁殖。同一基因型的分株呈聚集分布。在调查的所有种群中，种群内的平均多样性非常低（例如，0.111的Nei的基因多样性），与自然保护区的人口保持相对较高的遗传多样性。在所有的群体中，检测到高的遗传分化（AMOVA：ΦST= 0.500; Nei的遗传多样性：GST= 0.465，贝叶斯分析：ΦB= 0.436），以及隔离的距离模式。低幼苗招募由于近亲繁殖，限制基因流，遗传漂变的决定因素，负责低遗传多样性和高遗传分化观察。
Dysosma pleiantha, an important threatened medicinal plant species, is restricted in distribution to southeastern China. The species is capable of reproducing both sexually and asexually. In this study, inter-simple sequence repeat marker data were obtained and analyzed with respect to genetic variation and genetic structure. The extent of clonality, together with the clonal and sexual reproductive strategies, varied among sites, and the populations under harsh ecological conditions tended to have large clones with relatively low clonal diversity caused by vegetative reproduction. The ramets sharing the same genotype show a clumped distribution. Across all populations surveyed, average within-population diversity was remarkably low (e.g., 0.111 for Nei’s gene diversity), with populations from the nature reserves maintaining relatively high amounts of genetic diversity. Among all populations, high genetic differentiation (AMOVA: ΦST= 0.500; Nei’s genetic diversity:GST= 0.465, Bayesian analysis: ΦB= 0.436) was detected, together with an isolation-by-distance pattern. Low seedling recruitment due to inbreeding, restricted gene flow, and genetic drift are proposed as determinant factors responsible for the low genetic diversity and high genetic differentiation observed.