Effect of adipose-derived mesenchymal stem cell transplantation on vascular calcification in rats with adenine-induced kidney disease.
Effect of adipose-derived mesenchymal stem cell transplantation on vascular calcification in rats with adenine-induced kidney disease.
复制标题
DOI：
10.1038/s41598-017-14492-9
复制
发表时间：
2017-10-25
影响因子：
4.6
通讯作者：
Yokoo T
中科院分区：
文献类型：
--
作者：
Yokote S;Katsuoka Y;Yamada A;Ohkido I;Yokoo T
以前的研究已经研究了使用间充质干细胞（MSCs）治疗受损肾脏。然而，脂肪来源的间充质干细胞（ASCs）对慢性肾脏疾病（CKD）血管钙化的影响仍然知之甚少。在本研究中，我们探讨了ASCs治疗CKD和血管钙化的潜力。用含0.75%腺嘌呤的饲粮诱导雄性sd大鼠CKD 4周。与对照组相比，ASCs移植显著降低了血清无机磷（Pi）。肾脏组织病理学显示对照组肾小管腔扩张更大，间质纤维化更大。ASCs移植组主动脉钙、Pi含量均低于对照组。Von Kossa染色显示ASCs移植可抑制血管钙化。因此，本研究揭示了在CKD大鼠模型中，自体ASCs移植可抑制肾脏损伤并抑制血管钙化的进展，提示自体ASCs移植是一种预防CKD进展和血管钙化的新途径。
Previous studies have investigated the use of mesenchymal stem cells (MSCs) to treat damaged kidneys. However, the effect of adipose-derived MSCs (ASCs) on vascular calcification in chronic kidney disease (CKD) is still poorly understood. In the present study, we explored the potential of ASCs for the treatment of CKD and vascular calcification. CKD was induced in male Sprague-Dawley rats by feeding them a diet containing 0.75% adenine for 4 weeks. ASCs transplantation significantly reduced serum inorganic phosphorus (Pi) as compared to that in the control. The histopathology of the kidneys showed a greater dilation of tubular lumens and interstitial fibrosis in the control group. Calcium and Pi contents of the aorta in the ASCs transplantation group were lower than those in the control group. Von Kossa staining of the thoracic aorta media revealed that ASCs transplantation suppressed vascular calcification. Thus, this study revealed that autogenic ASCs transplantation inhibits kidney damage and suppresses the progression of vascular calcification in the CKD rat model, suggesting that autogenic ASCs transplantation is a novel approach for preventing the progression of CKD and vascular calcification.
登录
查看更多内容
影响因子：
168.9
作者：
Lozano, Rafael;Naghavi, Mohsen;Murray, Christopher J. L.
通讯作者：
Murray, Christopher J. L.
影响因子：
64.8
作者：
Takasato, Minoru;Er, Pei X.;Little, Melissa H.
通讯作者：
Little, Melissa H.
影响因子：
4.8
作者：
Byon, Chang Hyun;Javed, Amjad;Chen, Yabing
通讯作者：
Chen, Yabing
影响因子：
4.5
作者：
Anan, Hoda H.;Zidan, Rania A.;Abd-El Fattah, Enas A.
通讯作者：
Abd-El Fattah, Enas A.
影响因子：
6.2
作者：
Kramann, Rafael;Kunter, Uta;Schneider, Rebekka K.
通讯作者：
Schneider, Rebekka K.