A novel method to quantify the turnover and release of monocytes from the bone marrow using the thymidine analog 5′-bromo-2′-deoxyuridine
A novel method to quantify the turnover and release of monocytes from the bone marrow using the thymidine analog 5′-bromo-2′-deoxyuridine
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DOI：
10.1152/ajpcell.00035.2003
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发表时间：
2003-08-01
影响因子：
5.5
通讯作者：
van Eeden, SF
中科院分区：
文献类型：
--
作者：
Goto, Y;Hogg, JC;van Eeden, SF
本研究旨在开发使用胸苷类似物5 '-溴-2'-脱氧尿苷（BrdU）研究骨髓单核细胞产生和释放的方法。用BrdU（MOBrdU）标记骨髓中的分裂单核细胞，并使用双重免疫染色方法监测它们释放到血液中和从循环中消失。第一个MOBrdU出现在用BrdU标记后4小时的循环中，并在18小时达到峰值，此时标记了34.3 +/- 5.8%的单核细胞。在对照兔中，单核细胞通过骨髓的通过时间计算值为38.1 +/- 3.1 h，半衰期（T1/2）为12.7 h。将肺炎链球菌滴注到肺中加速了单核细胞从骨髓中的释放（在10小时达到峰值）并缩短了它们的骨髓通过时间（27.1 +/- 1.8对22.6 +/- 0.6，媒介物对肺炎; P < 0.05）。我们的结论是，这种非放射性同位素方法提供了一种新的方法来监测单核细胞动力学，并证实了以前的报告，即局灶性肺炎缩短单核细胞骨髓运输，并增加其释放到循环。
The present study was designed to develop methods to study the production and release of monocytes from the bone marrow using the thymidine analog 5'-bromo-2'-deoxyuridine (BrdU). Dividing monocytes in bone marrow were labeled with BrdU (MOBrdU), and their release into the blood and disappearance from the circulation were monitored using a double immunostaining method. The first MOBrdU appeared in the circulation 4 h after labeling with BrdU and peaked at 18 h when 34.3 +/- 5.8% of monocytes were labeled. The calculated transit time of monocytes through bone marrow was 38.1 +/- 3.1 h in control rabbits with a half-life (T1/2) of 12.7 h. Instillation of Streptococcus pneumoniae into the lung accelerated the release of monocytes from bone marrow (peak at 10 h) and shortened their bone marrow transit time (27.1 +/- 1.8 vs. 22.6 +/- 0.6, vehicle vs. pneumonia; P < 0.05). We conclude that this nonradioisotope method provides a novel way to monitor monocyte kinetics and confirmed previous reports that a focal pneumonia shortens monocyte marrow transit and increases their release into the circulation.