pH effects on micelle-water partitioning determined by micellar electrokinetic chromatography.
pH effects on micelle-water partitioning determined by micellar electrokinetic chromatography.
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DOI：
10.1016/s0021-9673(99)00599-3
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发表时间：
1999-08
期刊：
影响因子：
--
通讯作者：
D. J. Bailey;J. Dorsey
中科院分区：
文献类型：
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作者：
D. J. Bailey;J. Dorsey
在许多生物和环境条件下，水介质的pH值会发生变化，从而导致溶质分配的差异。然而，大多数生物分割和疏水性研究都是在pH为7的条件下进行的。利用胶束电动色谱法测定迁移因子，我们测定了pH值对19种化合物胶束-水分配的影响。我们对迁移因子方程进行了改进，修正后的苯胺迁移因子随pH值的降低而明显增加。校正后的迁移因子在研究的pH范围内对其余化合物是恒定的。我们还研究了五种胶束标记物，并确定癸烯酮是迄今为止最好的胶束标记物。癸烯酮具有很强的发色团，在所有pH水平下都可检测到，并且易于在流动相中溶解。
In many biological and environmental situations, the pH of aqueous media varies and differences in solute partitioning may result. However, the majority of biopartitioning and hydrophobicity studies conducted have been at pH 7. Using migration factors measured by micellar electrokinetic chromatography, we have determined pH effects on micelle–water partitioning for 19 compounds. We develop an improvement to the migration factor equation and the corrected migration factor for aniline shows a definite increase as pH decreases. The corrected migration factor was constant for the rest of the compounds over the pH ranged studied. We also investigated five micelle markers and determined that decanophenone is the best micelle marker to date. Decanophenone has a strong chromophore, detectable at all pH levels, and is easy to dissolve in the mobile phase.