In Vivo Rat Brain Imaging through Full-Field Optical Coherence Microscopy Using an Ultrathin Short Multimode Fiber Probe
In Vivo Rat Brain Imaging through Full-Field Optical Coherence Microscopy Using an Ultrathin Short Multimode Fiber Probe
复制标题
DOI：
10.3390/app9020216
复制
发表时间：
2019-01
期刊：
影响因子：
--
通讯作者：
M. Sato;Kai Eto;Junpei Masuta;Kenji Inoue;R. Kurotani;Hiroyuki Abe;I. Nishidate
中科院分区：
文献类型：
--
作者：
M. Sato;Kai Eto;Junpei Masuta;Kenji Inoue;R. Kurotani;Hiroyuki Abe;I. Nishidate
我们用纤芯直径为50μm、外径为125μm、长度为7.4 mm的超薄前向成像短多模光纤探头实现了全场光学相干显微镜。测得其轴向分辨率为2.14μm，横向分辨率为2.3μm。将4 mm的SMMF4 mm植入大鼠大脑皮质，扫描深度为147μm，视场为47μm，扫描深度约为20μm~90μm，根据三维图像的形态信息和图像信号的积分与插入长度的关系，给出了神经纤维的三维信息。
We demonstrate full-field optical coherence microscopy (OCM) using an ultrathin forward-imaging short multimode fiber (SMMF) probe with a core diameter of 50 μm, outer diameter of 125 μm, and length of 7.4 mm, which is a typical graded-index multimode fiber used for optical communications. The axial and lateral resolutions were measured to be 2.14 μm and 2.3 μm, respectively. By inserting the SMMF 4 mm into the cortex of an in vivo rat brain, scanning was performed to a depth of 147 μm from the SMMF facet with a field of view of 47 μm. Three-dimensional (3D) OCM images were obtained at depths ranging from approximately 20 μm to 90 μm. Based on the morphological information of the resliced 3D images and the dependence of the integration of the OCM image signal on the insertion length, the obtained 3D information of nerve fibers has been presented.