Biocompatibility and osseointegration of reconstituted keratin in an ovine model
Biocompatibility and osseointegration of reconstituted keratin in an ovine model
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DOI：
10.1002/jbm.a.32394
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发表时间：
2010-02-01
影响因子：
4.9
通讯作者：
Kelly, Robert J.
中科院分区：
文献类型：
--
作者：
Dias, George J.;Peplow, Philip V.;Kelly, Robert J.
重组角蛋白具有作为骨科应用原材料的潜力。本研究的目的是研究在绵羊模型中的角蛋白材料的体内生物相容性和骨整合。基于多孔或致密结构的角蛋白聚合物的六种不同修饰，通过中性或酸性处理再生，有或没有羟基磷灰石，制成小棒并插入绵羊股骨和胫骨中的钻孔圆形缺损中。对在LIP至术后24周的不同时间点采集的样品进行组织学检查。所有角蛋白植入物显示出相似的组织学特征，包括植入物周围和浸润的肉芽组织，随后是从现有骨辐射的新骨形成。到8周时，新骨已经生长到植入物表面的短距离内，并且在某些地方直接与角蛋白植入物贴壁。在12 - 24周期间，仅多孔结构存在外周骨小梁吸收和浸润。组织反应似乎模拟了相当惰性的材料。关于改善骨整合程度和加速重建角蛋白生物降解速率的进一步工作正在进行中。(C)2009 Wiley Periodicals，Inc.生物医学材料研究杂志92 A：513-520，2010
Reconstituted keratin has potential as a raw material for orthopaedic applications. The aim of this study was to investigate the in vivo biocompatibility and osseointegration of keratin materials in an ovine model. Six different modifications of the keratin polymer, based on porous or dense constructs, regenerated by either neutral or acidic treatment, with or Without hydroxyapatite, were made as small rods and inserted into drilled round defects in the femur and tibia of sheep. Histology was carried out on samples taken at different time points LIP to 24 weeks postsurgery. All keratin implants showed similar histological profiles, which included granulation tissue surrounding and infiltrating the implants, followed by new bone formation radiating from the existing bone. By 8 weeks, new bone had grown to within a short distance of the implant surface, and in some places was in direct apposition to the keratin implant. In the 12 to 24-week period, there was peripheral resorption and infiltration of bony trabeculae with regard to the porous constructs only. The tissue reaction appeared to model that of a fairly inert material. Further work on improving the extent of osseointegration and acceleration of the biodegradation rate of reconstituted keratin is underway. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 92A: 513-520, 2010