Formononetin Prevents Ovariectomy-Induced Bone Loss in Rats
Formononetin Prevents Ovariectomy-Induced Bone Loss in Rats
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DOI：
10.1007/s12272-010-0418-8
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发表时间：
2010-04-01
影响因子：
6.7
通讯作者：
Kim, Chungsook
中科院分区：
文献类型：
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作者：
Ha, Hyekyung;Lee, Ho Young;Kim, Chungsook
绝经后骨质疏松症的主要危险因素是雌激素缺乏。激素替代疗法对骨质疏松症有效，但它会引起一些显着的副作用。因此，在这项研究中，我们比较了三种植物雌激素的治疗效力：染料木素，大豆黄酮，芒柄花素。我们的结果表明，在Saos-2细胞中，芒柄花素和染料木素（5 × 10（-7）M）处理使碱性磷酸酶活性分别增加了33.0 +/- 5.8%和21.1 +/-4.0%。染料木黄酮抑制破骨细胞的形成呈剂量依赖性。在OVX大鼠中，1和10 mg/kg/天剂量的芒柄花素给药组显示胫骨内骨小梁面积（TBA）增加。染料木素和大豆苷元治疗组也显示胫骨TBA增加。所有给药组的腰椎TBA均高于对照组。总之，芒柄花素以及其他黄酮类化合物，如大豆苷元和染料木黄酮，抑制雌激素缺乏引起的骨丢失。
The major risk factor of postmenopausal osteoporosis is estrogen deficiency. Hormone replacement therapy is efficacious against osteoporosis, but it induces several significant adverse effects. In this study, therefore, we compared therapeutic potencies of three phytoestrogens: genistein, daidzein, and formononetin. Our result showed that in Saos-2 cells, formononetin and genistein (5 x 10(-7) M) treatment increased alkaline phosphatase activity by 33.0 +/- 5.8% and 21.1 +/- 4.0%. Genistein inhibited osteoclast formation in a dose-dependent manner. In OVX rats, formononetin-treated groups given 1 and 10 mg/kg/day displayed increased trabecular bone areas (TBAs) within the tibia. Genistein- and daidzein-treated groups also displayed increased tibial TBAs. TBAs of the lumbar vertebrae were higher in all treated groups than in the control group. In conclusion, formononetin as well as other isoflavones, such as daidzein and genistein, inhibited bone loss caused by estrogen-deficiency.