Auxin-induced changes in the molecular weight of hemicellulosic polysaccharides of the Avena coleoptile cell wall
Auxin-induced changes in the molecular weight of hemicellulosic polysaccharides of the Avena coleoptile cell wall
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生长素诱导的燕麦胚芽鞘细胞壁半纤维素多糖分子量的变化
DOI：
10.1093/oxfordjournals.pcp.a075934
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发表时间：
1979
影响因子：
4.9
通讯作者：
Y. Masuda
中科院分区：
文献类型：
--
作者：
N. Sakurai;K. Nishitani;Y. Masuda
用凝胶渗透色谱法和粘度法测定了燕麦胚芽鞘细胞壁水溶性半纤维素（半纤维素B）的平均分子量。对从GPC柱（Sepharose 4 B）洗脱的henucellulose B的中性糖组成的分析表明，其由具有高分子量的β-葡聚糖和具有低分子量的阿拉伯木聚糖组成。生长素对半纤维素B分子分布影响的动力学研究表明，生长素降低了β-葡聚糖含量的半纤维素早在第一个小时的孵育，但不是阿拉伯木聚糖含量，当它刺激胚芽鞘节的延伸。从色谱图计算的重均分子量表明，生长素降低半纤维素B的分子量，这也证实了粘度。因此，生长素可能通过影响细胞壁的厚度而引起细胞壁疏松，从而导致细胞伸长。葡聚糖降解或通过降低半纤维素B的分子量。
The average molecular weight of the water soluble hemicelluloses (hemicellulose B) of the Avena coleoptile cell wall was determined by gel permeation chromatography (GPC) and viscometry. Analysis of the neutral sugar composition of henucellulose B eluted from a GPC column (Sepharose 4B) indicated that it consists of /J-glucan with a high molecular weight and arabinoxylan with a low molecular weight. A kinetic study of the effect of auxin on the molecular distribution of henucellulose B demonstrated that auxin decreased the /?-glucan content of the hemicellulose as early as the first hour incubation, but not the arabinoxylan content, when it stimulated the extension of the coleoptile segments. Calculation of the weight-average molecular weight from the chromatograms suggested that auxin decreased the molecular weight of hemicellulose B; this was also confirmed by viscometry. Thus, auxin may cause cell wall loosening, leading to cell extension, through its effect on /?-glucan degradation or through the decrease in the molecular weight of hemicellulose B.