Human erythroid burst-forming units. Growth in vitro is dependent on monocytes, but not T lymphocytes.
Human erythroid burst-forming units. Growth in vitro is dependent on monocytes, but not T lymphocytes.
复制标题
人类红细胞爆发形成单位。
DOI：
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发表时间：
1981
影响因子：
15.9
通讯作者：
K. Zuckerman
中科院分区：
文献类型：
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作者：
K. Zuckerman
用含促红细胞生成素的血浆凝块培养人外周血单个核细胞亚群，研究单核细胞和T淋巴细胞在外周血红系形成单位(BFU-E)调节中的作用。单个核细胞中单核细胞耗尽(平均为预期的11%，范围为0~42%)或单核细胞和T细胞同时耗尽(平均为预期的6.5%，范围为0.5~12%)后，BFU-E生长显著降低。T细胞耗竭不影响BFU-E的体外生长。以10(5)个去单核细胞和去T淋巴细胞的单个核细胞为靶细胞(单核细胞不到1%，T细胞不到5%)，加入10(4)个单核细胞，BFU-E的生长恢复到预期的40%，单独加入10(5)个单核细胞，BFU-E的生长恢复到预期的96%。加入多达2×10(5)T细胞，但没有单核细胞，结果仅刺激了预期BFU-E生长的34%。加入不影响BFU-E生长的2×10(4)T细胞可显著增强5-20×10(3)单核细胞对BFU-E生长的刺激作用。因此，单核细胞似乎能够在促红细胞生成素存在的情况下刺激BFU-E的体外生长。T细胞也可以产生少量的BFU-E刺激物。然而，T淋巴细胞在体外调节BFU-E的最重要作用似乎更可能是与单核细胞相互作用的结果，以及刺激BFU-E生长的单核细胞生成增加的结果。
The roles of monocytes and T lymphocytes in the regulation of human peripheral blood erythroid burst-forming units (BFU-E) were studied in erythropoietin-containing plasma clot cultures of subpopulations of human blood mononuclear cells. BFU-E growth was decreased significantly after depletion of monocytes alone (mean 11% of expected, range 0 to 42% of expected) or depletion of both monocytes and T cells (mean 6.5% of expected, range 0.5 to 12% of expected) from mononuclear cells. T cell depletion did not impair BFU-E growth in vitro. Using 10(5) monocyte- and T lymphocyte-depleted mononuclear cells as target cells (less than 1% monocytes, less than 5% T cells), BFU-E growth was restored to 40% of expected by addition of 10(4) monocytes, and to 96% of expected by 10(5) monocytes alone. Addition of as many as 2 X 10(5) T cells but no monocytes resulted in stimulation to only 34% of expected BFU-E growth. Addition of 2 X 10(4) T cells, which alone did not affect BFU-E growth, could augment significantly the stimulatory effect of 5-20 X 10(3) monocytes on BFU-E growth. Thus, monocytes alone appear to be capable of stimulating BFU-E growth in vitro in the presence of erythropoietin. T cells also may make small quantities of BFU-E stimulators. However, it seems more likely that the most important role of T lymphocytes in BFU-E regulation in vitro is a result of interactions with monocytes and augmentation of monocyte production of stimulators of BFU-E growth.