Cellular Response of Anodized Titanium Surface by Poly(Lactide-co-Glycolide)/Bone Morphogenic Protein-2
Cellular Response of Anodized Titanium Surface by Poly(Lactide-co-Glycolide)/Bone Morphogenic Protein-2
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DOI：
10.1007/s13770-018-0137-7
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发表时间：
2018-10-01
影响因子：
3.6
通讯作者：
Heo, Seong-Joo
中科院分区：
文献类型：
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作者：
Lee, Su-Young;Koak, Jai-Young;Heo, Seong-Joo
背景技术背景：本研究的目的是检查用聚乙烯处理的阳极化钛的物理特性和初始生物学特性方法：制备钛试件，NC组为对照组，NC组为300 V阳极氧化组，NC组为300 V阳极氧化组; PC组：阳极氧化后滴加0.02 ml PLG溶液，D组：阳极氧化后滴加0.02 ml PLG/rhBMP-2溶液，D组：阳极氧化后滴加0.02 ml PLG/rhBMP-2溶液，D组：阳极氧化后滴加0.02 ml PLG/rhBMP-2溶液（3.75 μ g/盘）; E组阳极氧化，然后通过电喷雾用0.02 ml PLG/rhBMP-2（3.75 μ g/盘）涂覆。培养人成骨细胞样肉瘤细胞。细胞增殖和碱性磷酸酶（ALP）活性测定。逆转录-聚合酶链反应检测Runx-2基因。结果：3 d后，rhBMP-2负载的钛盘与未负载rhBMP-2的钛盘相比，成骨细胞增殖明显增强。在rhBMP-2负载的钛盘上的ALP 4活性显著高于rhBMP-2未负载的钛盘。结论：PLG聚合物与rhBMP-2复合后可促进钛表面细胞的增殖、分化及成骨蛋白的形成。
BACKGROUND: The purpose of this study is to examine physical characteristics of and initial biological properties to anodized titanium treated with poly(D, L-lactide-co-glycolide) (PLG) mixed with recombinant human bone morphogenic protein-2 (rhBMP-2).METHODS: Titanium specimens were prepared in groups of four as follows: group NC was anodized under 300 V as control; group PC was anodized then dropped and dried with solution 0.02 ml PLG; group D was anodized then dropped and dried with solution 0.02 ml PLG/rhBMP-2 (3.75 mu g per disc); and group E was anodized then coated with 0.02 ml PLG/rhBMP-2 (3.75 mu g per disc) by electrospray. Human osteoblastic-like sarcoma cells were cultured. Cell proliferation and alkaline phosphatase (ALP) activity test were carried out. Runx-2 gene was investigated by the reverse transcription-polymerase chain reaction. Immunofluorescence outcome of osteogenic proteins was observed.RESULTS: After 3 days, there were significantly higher proliferations compared rhBMP-2 loaded titanium discs with rhBMP-2 unloaded discs. The ALPase activity on rhBMP-2 loaded titanium discs was significantly higher than in rhBMP-2 unloaded discs. The expression level of Runx2 mRNA presented the highest on the PLG/rhBMP-2-coated surface.CONCLUSION: PLG polymers mixed with rhBMP-2 might improve proliferation, differentiation and osteogenic protein formation of cells on the anodized titanium.