Genetic analysis of mating locus linked mutations in Chlamydomonas reinhardii.

Genetic analysis of mating locus linked mutations in Chlamydomonas reinhardii.
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莱茵衣藻交配位点相关突变的遗传分析。

DOI:
10.1093/genetics/111.3.447
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发表时间:
1985
期刊:
影响因子:
3.3
通讯作者:
Goodenough,UW
Goodenough,UW
中科院分区:
生物学2区
文献类型:
--
作者:
Galloway,RE;Goodenough,UW

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用4株突变株(imp-1、imp-10、imp-11和imp-12)对莱氏衣原体的交配型(mt)位点进行了分析。所有这些基因都已经被证明,或者在这里被证明,携带与正(mt+)或负(mt-)基因座相关的突变,它们在互补测试中的行为使我们能够为每个基因座定义几个不同的功能。具体来说,我们建议,themt+基因座包含以下基因或调控元件:一个位点指定dsfu,这是必要的配子之间的性融合;一个位点指定dupp(单亲加),这控制叶绿体基因遗传方面,也许也受精卵成熟;和一个位点指定SAD,这在性粘附功能。该emt基因座还包含asad基因座以及一个名为mid的基因或调节元件,这是MT +/MT-二倍体中负显性所必需的。
The mating-type (mt) locus ofChlamydomonas reinhardiihas been analyzed using four mutant strains (imp-1, imp-10, imp-11andimp-12). All have been shown, or are shown here, to carry mutations linked to either the plus (mt+) or the minus (mt-) locus, and their behavior in complementation tests has allowed us to define several distinct functions for each locus. Specifically, we propose that themt+locus contains the following genes or regulatory elements: a locus designatedsfu, which is necessary for sexual fusion between gametes; a locus designatedupp(uniparental plus), which controls aspects of chloroplast gene inheritance and perhaps also zygote maturation; and a locus designatedsad, which functions in sexual adhesion. Themt-locus also contains asadlocus as well as a gene or regulatory element designatedmid, which is necessary for the minus dominance inmt+/mt-diploids.