Direct observation of interaction between proteins and blood-compatible polymer surfaces
Direct observation of interaction between proteins and blood-compatible polymer surfaces
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DOI：
10.1116/1.2794712
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发表时间：
2007-12-01
期刊：
影响因子：
2.1
通讯作者：
Hara, Masahiko
中科院分区：
文献类型：
--
作者：
Hayashi, Tomohiro;Tanaka, Masaru;Hara, Masahiko
用原子力显微镜测量了血液相容性高分子（聚（2-甲氧基乙基丙烯酸酯：PMEA））与蛋白质（纤维蛋白原和牛血清白蛋白（BSA））之间的粘附力。PMEA表面几乎没有显示出对天然蛋白质分子的粘附，而非血液相容性的聚（丙烯酸正丁酯）：PBA强烈地粘附于蛋白质。有趣的是，当蛋白质变性时，PMEA与蛋白质之间确实出现粘附。在所有情况下，这些趋势不受溶液条件的影响。结合先前的报道，作者得出结论，界面水分子在PMEA的蛋白质抗性中起着关键作用。(c)2007年美国真空学会。
The adhesion force between blood-compatible polymer (poly(2- methoxyethyl acrylate: PMEA) and proteins ( fibrinogen and bovine serum albumin (BSA)) were measured by atomic force microscopy. The PMEA surface showed almost no adhesion to native protein molecules, whereas non-blood- compatible poly (n- butyl acrylate): PBA strongly adhered to proteins. Interestingly, adhesion did appear between PMEA and proteins when the proteins were denatured. In all cases, these trends were not affected by the conditions of the solution. Combining the results with previous reports, the authors conclude that interfacial water molecules play a critical role in the protein resistance of PMEA. (c) 2007 American Vacuum Society.