Vertical Changes in Bacterial Communities in Percolating Water of a Japanese Paddy Field as Revealed by PCR-DGGE
Vertical Changes in Bacterial Communities in Percolating Water of a Japanese Paddy Field as Revealed by PCR-DGGE
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DOI：
10.1111/j.1747-0765.2005.tb00010.x
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发表时间：
2005-02
影响因子：
2
通讯作者：
J. Murase;Miho Shimizu;M. Hayashi;K. Matsuya;M. Kimura
中科院分区：
文献类型：
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作者：
J. Murase;Miho Shimizu;M. Hayashi;K. Matsuya;M. Kimura
从日本稻田的耕层和底土层中采集渗滤水样品，通过聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）靶向部分16S rRNA基因并随后测序，将细菌群落与洪水进行比较。DGGE条带数在16~28条之间，不同采样点和采样时间差异不显著。三种来源的水样只有2条共有带。DGGE分析结果表明，洪水和耕层灌溉水的DGGE谱带具有一定的特异性，而底土灌溉水的DGGE谱带与洪水和耕层灌溉水的DGGE谱带没有明显的特异性，但有7条和1条相同的谱带。DGGE带型聚类分析表明，耕层土壤水样中的DGGE带型聚类明显，其它水样之间的DGGE带型关系较近。这些结果表明，土壤微生物群落的变化过程中下降的水通过犁层和底土层。从犁耕层的净化水样品的DGGE条带的序列显示出与厌氧细菌，如铁还原剂或未培养的细菌DNA分离的环境中，被认为是少氧的密切关系。另一方面，来自底土层的洪水和排水样品的特异性条带的序列显示与从淡水环境中分离的未培养细菌DNA的密切关系。
Percolating water was sampled from the plow layer and subsoil layer in a Japanese paddy field, and the bacterial communities were compared together with floodwater by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) targeting a partial 16S rRNA gene and subsequent sequencing. The number of DGGE bands ranged from 16 to 28 with no significant differences among the sampling sites and times. Only 2 bands were common for the three sources of water samples. DGGE bands specific for the floodwater samples and percolating water samples from the plow layer were identified, while percolating water samples from the subsoil layer did not show specific bands but displayed common bands to those of the floodwater samples (7 bands) and percolating water samples from the plow layer (1 band). Cluster analysis of the DGGE banding patterns showed a distinct clustering in the samples of percolating water from the plow layer and a closer relationship between the others. These results suggest that the bacterial communities in percolating water changed during downward movement through the plow layer and subsoil layer. Sequences of the DGGE bands specific for the samples of percolating water from the plow layer showed a close relationship with anaerobic bacteria such as iron-reducers or uncultured bacterial DNA isolated from environments that are considered to be less oxic. On the other hand, the sequences of the bands specific for the samples of floodwater and percolating water from the subsoil layer showed a close relationship with uncultured bacterial DNA isolated from freshwater environments.