Isolation of gametes and zygotes from Setaria viridis
Isolation of gametes and zygotes from Setaria viridis
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DOI：
10.1007/s10265-022-01393-w
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发表时间：
2021-11
影响因子：
2.8
通讯作者：
Erika Toda;Takatoshi Kiba;Norio Kato;T. Okamoto
中科院分区：
文献类型：
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作者：
Erika Toda;Takatoshi Kiba;Norio Kato;T. Okamoto
狗尾草(Setaria Viridis)是谷子(Setaria Italica)的野生祖先，是C4作物的有效模式植物。病毒具有几个令人满意的特征，如世代时间短，多产种子和小基因组。这些优势非常适合于研究被子植物，特别是C4作物物种的分子机制。在这里，我们报告了一种从配子和受精卵中分离配子和受精卵的方法。病毒花。为了分离卵细胞，从未授粉的成熟花朵中采集子房，并横向切开，这样就可以直接进入胚囊。此后，从被剥离的卵巢的基底部的切端释放卵细胞。为了分离精子细胞，从花药中释放的花粉粒被浸泡在甘露醇溶液中，导致花粉粒破裂，从而释放精子细胞。此外，还成功地从新鲜授粉的花朵中分离到了S.分离的受精卵在液体培养基中发育成球形胚胎和细胞团。因此，隔离的S。病毒配子、受精卵和胚胎可用于详细观察和研究被子植物的受精和发育事件。
Setaria viridis, the wild ancestor of foxtail millet (Setaria italica), is an effective model plant for larger C4crops becauseS. viridishas several desirable traits, such as short generation time, prolific seed production and a small genome size. These advantages are well suited for investigating molecular mechanisms in angiosperms, especially C4crop species. Here, we report a procedure for isolating gametes and zygotes fromS. viridisflowers. To isolate egg cells, ovaries were harvested from unpollinated mature flowers and cut transversely, which allowed direct access to the embryo sac. Thereafter, an egg cell was released from the cut end of the basal portion of the dissected ovary. To isolate sperm cells, pollen grains released from anthers were immersed in a mannitol solution, resulting in pollen-grain bursting, which released sperm cells. Additionally,S. viridiszygotes were successfully isolated from freshly pollinated flowers. Isolated zygotes cultured in a liquid medium developed into globular-like embryos and cell masses. Thus, isolatedS. viridisgametes, zygotes and embryos are attainable for detailed observations and investigations of fertilization and developmental events in angiosperms.