Chromosomal distribution of Heterochromatin Protein 1 (HP1) in Drosophila:: a cytological map of euchromatic HP1 binding sites
Chromosomal distribution of Heterochromatin Protein 1 (HP1) in Drosophila:: a cytological map of euchromatic HP1 binding sites
复制标题
DOI：
10.1023/a:1022971407290
复制
发表时间：
2003-03-01
期刊：
影响因子：
1.5
通讯作者：
Pimpinelli, S
中科院分区：
文献类型：
--
作者：
Fanti, L;Berloco, M;Pimpinelli, S
异染色质蛋白1（Heterochromatin Protein 1，HP 1）是一种保守的蛋白质，它与果蝇异染色质密切相关。我们以前证明了HP 1的另一个重要特性是其定位于果蝇的端粒，这一特征反映了其作为端粒加帽蛋白的关键功能。在这里，我们报告我们的分析常染色质的网站，HP 1本地化。使用抗HP 1抗体，我们比较了Ore-R野生型实验室菌株和四个不同的自然种群的多线染色体上的免疫染色模式。HP 1被发现积累在特定的常染色质位点，与一个子集的站点之间的菌株保守。这些位点似乎不是由已知重复DNA的富集所定义的。几种果蝇之间的HP 1模式的比较显示，与特定的常染色质区域，异染色质和端粒的协会是一个保守的特征HP 1。基于这些结果，我们认为HP 1的功能比通常假设的更广泛。除了它在异染色质组装和端粒稳定性中的作用外，我们提出HP 1在调节许多不同常染色质区域的表达中起重要作用。
The Heterochromatin Protein 1 (HP1) is a conserved protein which is best known for its strong association with the heterochromatin of Drosophila melanogaster. We previously demonstrated that another important property of HP1 is its localization to the telomeres of Drosophila, a feature that reflects its critical function as a telomere capping protein. Here we report our analysis of the euchromatic sites to which HP1 localizes. Using an anti-HP1 antibody, we compared immunostaining patterns on polytene chromosomes of the Ore-R wild type laboratory strain and four different natural populations. HP1 was found to accumulate at specific euchromatic sites, with a subset of the sites conserved among strains. These sites do not appear to be defined by an enrichment of known repetitive DNAs. Comparisons of HP1 patterns among several Drosophila species revealed that association with specific euchromatic regions, heterochromatin and telomeres is a conserved characteristic of HP1. Based on these results, we argue that HP1 serves a broader function than typically postulated. In addition to its role in heterochromatin assembly and telomere stability, we propose that HP1 plays an important role in regulating the expression of many different euchromatic regions.