Detection of colonies of Bacteroides gingivalis by a rapid fluorescence assay for trypsin-like activity.
Detection of colonies of Bacteroides gingivalis by a rapid fluorescence assay for trypsin-like activity.
复制标题
通过快速荧光测定胰蛋白酶样活性来检测牙龈拟杆菌菌落。
DOI：
10.1111/j.1399-302x.1987.tb00279.x
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发表时间：
1987
影响因子：
--
通讯作者：
Slots,J
中科院分区：
文献类型：
--
作者：
Slots,J
评估了合成荧光化合物苯甲氧基-L-精氨酸-7-氨基-4-甲基香豆素酰胺-HC1 (CAAM) 检测牙龈拟杆菌菌落中胰蛋白酶样活性的能力。CAAM 与测试的所有牙龈拟杆菌分离株均发出荧光，并且不与其他人类黑色素拟杆菌物种发生反应。 CAAM 测试用于快速推定鉴定 B 的实用性。讨论了牙龈炎。
The synthetic fluorogenic compound carbobenzoxy‐L‐arginine‐7‐amino‐4‐me‐thylcoumarin amide‐HC1 (CAAM) was evaluated for its ability to detect trypsin‐like activity in colonies ofBacteroides gingivalis.CAAM fluoresced with all B. gingivalis isolates tested and did not react with other human black‐pigmented bacteroides species. The utility of the CAAM test for rapid presumptive identification ofB. gingivalisis discussed.