Gut bioengineering strategies for regenerative medicine.
Gut bioengineering strategies for regenerative medicine.
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DOI：
10.1152/ajpgi.00206.2020
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发表时间：
2020-11
期刊：
影响因子：
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通讯作者：
John D. O’Neill;M. Pinezich;Brandon A. Guenthart;G. Vunjak‐Novakovic
中科院分区：
文献类型：
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作者：
John D. O’Neill;M. Pinezich;Brandon A. Guenthart;G. Vunjak‐Novakovic
胃肠道疾病负担在美国和世界范围内持续上升。开发生物工程策略来模拟肠道损伤或疾病并重建功能性肠道组织可以扩大治疗选择并改善临床结果。目前的方法利用了快速发展的肠道生物工程工具包，其目的是：（i）在微组织模型或可植入移植物的多个尺度下从头产生肠道样组织，以及（ii）体内功能性肠道的再生。虽然在肠道类器官培养和工程组织方面取得了重大进展，但预测性体外模型和有效再生疗法的开发仍然具有挑战性。在这篇综述中，我们调查了新兴的生物工程工具和最新的方法学进展，以确定未来的机遇和挑战，在肠道生物工程疾病建模和再生医学。
Gastrointestinal disease burden continues to rise in the United States and worldwide. The development of bioengineering strategies to model gut injury or disease and to re-establish functional gut tissue could expand therapeutic options and improve clinical outcomes. Current approaches leverage a rapidly evolving gut bioengineering toolkit aimed at: (i) de-novo generation of gut-like tissues at multiple scales for microtissue models or implantable grafts, and (ii) regeneration of functional gut in vivo. Although significant progress has been made in intestinal organoid cultures and engineered tissues, development of predictive in-vitro models and effective regenerative therapies remains challenging. In this review, we survey emerging bioengineering tools and recent methodological advances to identify future opportunities and challenges in gut bioengineering for disease modeling and regenerative medicine.