A model of host-microbial interactions in an open mammalian ecosystem
A model of host-microbial interactions in an open mammalian ecosystem
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DOI：
10.1126/science.273.5280.1380
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发表时间：
1996-09-06
期刊：
影响因子：
56.9
通讯作者：
Gordon, JI
中科院分区：
文献类型：
--
作者：
Bry, L;Falk, PG;Gordon, JI
哺乳动物肠道中存在的复杂微生物种群的维持和意义还知之甚少。对常规饲养和无细菌饲养的NMRI小鼠的比较表明，在小肠上皮中产生岩藻糖化糖偶联物和α1，2岩藻糖基转移酶信使RNA需要正常的微生物群。在无菌小鼠身上，这种菌群的一种成分--丝状拟杆菌能恢复岩藻糖基化程序，而带有转座子插入的同基因菌株则不能恢复其利用L岩藻糖作为碳源的能力。像这样的简化模型应该有助于开放微生物生态系统的研究。
The maintenance and significance of the complex populations of microbes present in the mammalian intestine are poorly understood. Comparison of conventionally housed and germ-free NMRI mice revealed that production of fucosylated glycoconjugates and an alpha 1,2-fucosyltransferase messenger RNA in the small-intestinal epithelium requires the normal microflora. Colonization of germ-free mice with Bacteroides thetaiotadmicron, a component of this flora, restored the fucosylation program, whereas an isogenic strain carrying a transposon insertion that disrupts its ability to use L-fucose as a carbon source did not. Simplified models such as this should aid the study of open microbial ecosystems.