Wrinkled1 Accelerates Flowering and Regulates Lipid Homeostasis between Oil Accumulation and Membrane Lipid Anabolism in Brassica napus.
Wrinkled1 Accelerates Flowering and Regulates Lipid Homeostasis between Oil Accumulation and Membrane Lipid Anabolism in Brassica napus.
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DOI：
10.3389/fpls.2015.01015
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发表时间：
2015
影响因子：
5.6
通讯作者：
Hong Y
中科院分区：
文献类型：
--
作者：
Li Q;Shao J;Tang S;Shen Q;Wang T;Chen W;Hong Y
WRI1属于APETALA2转录因子家族，是植物特有的，是拟南芥油脂合成的中心调节因子。WRI 1对植物尤其是农作物的脂类综合代谢和生长发育的影响尚不清楚。这项研究发现，BnWRI 1在甘蓝型油菜中加速开花并增强种子和叶片中的油积累，而不会导致可见的生长抑制。BnWRI 1减少了储存碳水化合物，增加了可溶性糖，以促进碳通量到脂质的增加。BnWRI 1定位于细胞核，并直接结合到参与脂肪酸（FA）合成和脂质组装的基因的近端上游区域的AW盒。BnWRI1的过表达（OE）导致参与糖酵解、FA合成、脂质组装和开花的基因的上调。脂质分析显示，增加半乳糖脂单半乳糖基二酰基甘油（MGDG），双半乳糖基二酰基甘油（DGDG），和磷脂酰胆碱（PC）在OE植物的叶子，而它表现出降低水平的半乳糖脂DGDG和MGDG和增加水平的PC，磷脂酰乙醇酰胺，和油[三酰甘油（TAG）]在OE植物的角果在种子发育早期阶段。这些结果表明，BnWRI 1是重要的TAG，膜脂和糖之间的稳态，从而促进开花和油的积累在B。油菜。
Wrinkled1 (WRI1) belongs to the APETALA2 transcription factor family; it is unique to plants and is a central regulator of oil synthesis in Arabidopsis. The effects of WRI1 on comprehensive lipid metabolism and plant development were unknown, especially in crop plants. This study found that BnWRI1 in Brassica napus accelerated flowering and enhanced oil accumulation in both seeds and leaves without leading to a visible growth inhibition. BnWRI1 decreased storage carbohydrates and increased soluble sugars to facilitate the carbon flux to lipid anabolism. BnWRI1 is localized to the nucleus and directly binds to the AW-box at proximal upstream regions of genes involved in fatty acid (FA) synthesis and lipid assembly. The overexpression (OE) of BnWRI1 resulted in the up-regulation of genes involved in glycolysis, FA synthesis, lipid assembly, and flowering. Lipid profiling revealed increased galactolipids monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), and phosphatidylcholine (PC) in the leaves of OE plants, whereas it exhibited a reduced level of the galactolipids DGDG and MGDG and increased levels of PC, phosphatidylethanolamide, and oil [triacylglycerol (TAG)] in the siliques of OE plants during the early seed development stage. These results suggest that BnWRI1 is important for homeostasis among TAG, membrane lipids and sugars, and thus facilitates flowering and oil accumulation in B. napus.