DIABETES AFFECTS ALPHA-CRYSTALLIN CHAPERONE FUNCTION
DIABETES AFFECTS ALPHA-CRYSTALLIN CHAPERONE FUNCTION
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DOI：
10.1006/bbrc.1995.1954
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发表时间：
1995-07-06
影响因子：
3.1
通讯作者：
ABRAHAM, EC
中科院分区：
文献类型：
--
作者：
CHERIAN, M;ABRAHAM, EC
体外糖基化先前已被证明影响α -晶体蛋白伴侣蛋白的功能。在目前的研究中，我们发现这种功能在糖尿病中受损。用凝胶渗透色谱法从链脲佐菌素-糖尿病大鼠和年龄匹配的正常大鼠的水溶性蛋白中分离出α (H)、α (L)和总α (H)。基于β (L)-结晶蛋白热变性实验，糖尿病大鼠的伴侣蛋白功能明显降低。(C) 1995学术出版社，Inc。
rn vitro glycation was previously shown to influence alpha-crystallin chaperone function. In the present study we show that this function is compromised in diabetes. The alpha(H), alpha(L) and the total alpha fractions were isolated by gel permeation chromatography from the water - soluble protein of streptozotocin - diabetic as well as age-matched normal rats. Based on the beta(L)-crystallin thermal denaturation assay the chaperone function was significantly decreased in the diabetic rats. (C) 1995 Academic Press, Inc.