Prostaglandin E2 in basal gastric secretion and during stimulation of muscarinic receptors in man. Measurements with gas chromatography--mass spectrometry.
Prostaglandin E2 in basal gastric secretion and during stimulation of muscarinic receptors in man. Measurements with gas chromatography--mass spectrometry.
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前列腺素 E2 在基础胃分泌和刺激人毒蕈碱受体过程中。
DOI：
10.1111/j.1748-1716.1984.tb07547.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
C. Johansson
中科院分区：
文献类型：
--
作者：
A. Aly;K. Gréen;C. Johansson
胃粘膜中前列腺素E2（PGE 2）生物合成的生理重要性尚不清楚。已经提出了内源性洋地黄素在保护胃肠道上皮细胞中的作用，但是证据不足，并且很少通过同时测量PG产生来支持。无创取样胃内容物中PGE 2的量可反映体内胃粘膜中PGE 2的合成。为了证实早期报道的用放射免疫测定法进行的测量，我们用气相色谱-质谱法（GC-MS）测量了5名健康男性在基础条件下和用iv刺激毒蕈碱受体期间的胃液中的PGE 2。据报道，氨甲酰甲胆碱在狗中可增强PGE 2的输出。在所有基础样本中检测到PGE 2。输出的中位数为32.1 pmol/15 min（范围17.0-105.4，1 pmol = 0.352 ng），与早期研究的结果相似。氨甲酰胆碱输注（60微克。kg-1 . h-1）并不影响PGE 2的输出系统，尽管在输出的酸和氯化物的显着增加。毒蕈碱受体的刺激似乎不影响体内胃粘膜中PGE 2的合成。或者，PGE 2合成的变化可能被PGE 2的快速化学或酶降解或重吸收所掩盖。目前正在研究这些现象。
The physiological importance of prostaglandin E2 (PGE2) biosynthesis in the gastric mucosa is unknown. A role of endogenous prostaglandins in protecting the gastrointestinal epithelia has been suggested, but the evidence is insufficient and rarely supported by concomitant measurement of PG production. Amounts of PGE2 in luminal gastric contents which can be sampled atraumatically may reflect PGE2 synthesis in the gastric mucosa in vivo. To confirm earlier reported measurements made with radioimmunoassay we have measured by gas chromatography - mass spectrometry (GC-MS) PGE2 in gastric juice of five healthy men under basal conditions and during stimulation of muscarinic receptors with iv. bethanechol which in dog is reported to enhance PGE2 output. PGE2 was detected in all basal samples. The output was in median 32.1 pmol/15 min (range 17.0-105.4, 1 pmol = 0.352 ng), which is similar to results from earlier studies. Bethanechol infusion (60 micrograms . kg-1 . h-1) did not affect PGE2 outputs systematically in spite of a significant increase in outputs of acid and chlorides. Stimulation of muscarinic receptors does not seem to influence PGE2 synthesis in gastric mucosa in vivo. Alternatively changes in PGE2 synthesis may be masked by rapid chemical or enzymatical degradation or reabsorption of PGE2. Studies are under way to explore those phenomena.
氨胆碱（尿胆碱）对正常人和十二指肠溃疡患者胃酸和非顶叶分泌的影响。
影响因子：
29.4
作者：
Feldman,M;Schiller,L
通讯作者：
Schiller,L
钙和氨甲酰胆碱刺激大鼠结肠粘膜前列腺素合成：与环核苷酸代谢改变的关系。
DOI：
10.1016/0090-6980(81)90197-0
发表时间：
1981
期刊：
Prostaglandins
影响因子：
--
作者：
Craven,PA;DeRubertis,FR
通讯作者：
DeRubertis,FR