DEMONSTRATION OF CELL-TYPES AMONG CONE BIPOLAR NEURONS OF CAT RETINA
DEMONSTRATION OF CELL-TYPES AMONG CONE BIPOLAR NEURONS OF CAT RETINA
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DOI：
10.1098/rstb.1990.0201
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发表时间：
1990-12-29
影响因子：
6.3
通讯作者：
STERLING, P
中科院分区：
文献类型：
--
作者：
COHEN, E;STERLING, P
我们在一个视网膜的一小片中鉴定了所有的锥双极细胞（80个），然后详细研究了将轴突发送到内丛状层的亚板层B的完整子集（42个）。问题的关键是要了解以前提出的“类型”，基于大量人口的一些例子，是否可以得到证实，或者是否会有中间形式。将来自中央区域（1度偏心率）的组织制备为一系列279个切片，并在电子显微镜下拍照。13个细胞完全重建，并根据外部形态分为五类（b1-b5）。对于其中的九个细胞（两个来自类别b1-b4，一个来自类别b5），大多数突触输入和输出都被识别出来。当这九个细胞根据它们的突触模式平行排列时，它们被分为相同的五类。群体中剩余的29个细胞，虽然没有重建，但通过追踪它们在系列中的过程进行了详细研究。这些细胞中有10个是不完整的，它们靠近系列的边缘。其他19个细胞的形态和突触模式的分布基本上相同的子集研究总重建：当绘制在多参数空间，它们形成了不同的集群对应的五个形态类别。没有任何中间形式的迹象。群体中的所有神经元都被分类到某个簇中（没有中间形式），并且每个神经元都根据不同的标准被分类到同一个簇中，这表明这些簇代表了自然类型。每种类型在研究区域中形成一个规则阵列，其轴突“覆盖因子”接近1。
We identified all the cone bipolar cells (80) in a small patch of one retina and then studied in detail the complete subset (42) that sends axons to sublamina b of the inner plexiform layer. The point was to learn whether the 'types' suggested previously, based on a few examples from a large population, could be substantiated or whether there would be intermediate forms. Tissue from the area centralis (1-degrees eccentricity), was prepared as a series of 279 ultrathin sections and photographed in the electron microscope. Thirteen cells were reconstructed completely and paracelled into five categories (b1-b5) based on external morphology. For nine of these cells (two from categories b1-b4 and one from b5) most of the synaptic inputs and outputs were identified. When these nine cells were paracelled according to their synaptic patterns, they sorted into the same five categories. The remaining 29 cells in the population, though not reconstructed, were studied in detail by tracing their processes through the series. Ten of these cells, those near the margin of the series, were incomplete. The other 19 cells had essentially the same distribution of morphologies and synaptic patterns as the subset studied by total reconstruction: when plotted in multiparametric space, they formed distinct clusters corresponding to the five morphological categories. There was no hint of intermediate forms. That all the neurons in the population sort into some cluster (no intermediate forms), and that each neuron sorts into the same cluster by different criteria, argues that the clusters represent natural types. Each type forms a regular array in the region studied with an axonal 'coverage factor' that is close to one.