Protein activator of cyclic 3':5'-nucleotide phosphodiesterase of bovine or rat brain also activates its adenylate cyclase.
Protein activator of cyclic 3':5'-nucleotide phosphodiesterase of bovine or rat brain also activates its adenylate cyclase.
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牛或大鼠脑的环状3:5-核苷酸磷酸二酯酶的蛋白质激活剂也激活其腺苷酸环化酶。
DOI：
10.1016/0006-291x(75)90747-0
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发表时间：
1975
影响因子：
3.1
通讯作者：
E.Ann Tallant
中科院分区：
文献类型：
--
作者：
W. Y. Cheung;W. Y. Cheung;L. S. Bradham;Thomas J. Lynch;Ying Ming Lin;E.Ann Tallant
用Lubrol PX增溶的牛或大鼠脑腺苷酸环化酶（EC 4.6.1.1）含有一种激活剂，该激活剂通过阴离子交换树脂柱与酶分离。从腺苷酸环化酶的激活剂的解离使酶活性降低，并与外源性激活剂重建恢复充分的酶活性。从牛脑中分离出的环状3 '：5'-核苷酸磷酸二酯酶的纯蛋白激活剂（EC 3.1.4.17）也刺激了这种腺苷酸环化酶。激活剂对腺苷酸环化酶的刺激需要Ca++，其作用是立即和可逆的。虽然激活剂是特异性的，但它缺乏组织特异性;从牛脑中分离的激活剂有效地交叉激活大鼠腺苷酸环化酶，反之亦然。这些发现表明，脑腺苷酸环化酶需要激活剂来激活，并且该激活剂在功能上与磷酸二酯酶的蛋白质激活剂相同（J.B.C.249：4943-4954，1974）。
Bovine or rat brain adenylate cyclase (EC 4.6.1.1) solubilized by Lubrol PX contained an activator which was separated from the enzyme by an anionic exchange resin column. Dissociation of the activator from adenylate cyclase rendered the enzyme less active, and reconstituting with an exogenous activator restored full enzyme activity. A pure protein activator of cyclic 3′:5′-nucleotide phosphodiesterase (EC 3.1.4.17) isolated from bovine brain also stimulated this adenylate cyclase. Stimulation of adenylate cyclase by the activator required Ca++, the effect being immediate and reversible. Although the activator was specific, it lacked tissue specificity; an activator isolated from bovine brain cross-activated effectively adenylate cyclase from rat, and vice versa. These findings indicate that brain adenylate cyclase required an activator for activity and that this activator is functionally identical to the protein activator of phosphodiesterase (J.B.C.249: 4943–4954, 1974).