EGF Enhances Oligodendrogenesis from Glial Progenitor Cells.
EGF Enhances Oligodendrogenesis from Glial Progenitor Cells.
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EGF 增强神经胶质祖细胞的少突胶质细胞生成
DOI：
10.3389/fnmol.2017.00106
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发表时间：
2017
影响因子：
4.8
通讯作者：
Qiu M
中科院分区：
文献类型：
--
作者：
Yang J;Cheng X;Qi J;Xie B;Zhao X;Zheng K;Zhang Z;Qiu M
越来越多的证据表明表皮生长因子（EGF）信号在髓鞘发育和修复中起着积极的作用，但很少有人知道它对少突胶质细胞（OL）谱系细胞的早期生成和分化的生物学作用。在这项研究中，我们研究了EGF在早期OL发展中的作用与孤立的胶质限制性前体（GRP）细胞。研究发现，EGF与血小板衍生生长因子-AA（PDGFaa）合作促进GRP细胞的存活和自我更新，但在缺乏或PDGFaa的情况下，GRP细胞倾向于发育为O 4-早期少突胶质细胞前体细胞（OPCs）。在OPCs中，EGF与PDGFaa协同作用以维持其O 4阴性抗原表型。在PDGFaa撤除后，EGF通过减少凋亡和增加成熟OL的数量来促进OPCs的终末分化。总之，这些数据表明，表皮生长因子是一个重要的有丝分裂原，以提高少突胶质细胞的发展。
Emerging evidence indicates that epidermal growth factor (EGF) signaling plays a positive role in myelin development and repair, but little is known about its biological effects on the early generation and differentiation of oligodendrocyte (OL) lineage cells. In this study, we investigated the role of EGF in early OL development with isolated glial restricted precursor (GRP) cells. It was found that EGF collaborated with Platelet Derived Growth Factor-AA (PDGFaa) to promote the survival and self-renewal of GRP cells, but predisposed GRP cells to develop into O4− early-stage oligodendrocyte precursor cells (OPCs) in the absence of or PDGFaa. In OPCs, EGF synergized with PDGFaa to maintain their O4 negative antigenic phenotype. Upon PDGFaa withdrawal, EGF promoted the terminal differentiation of OPCs by reducing apoptosis and increasing the number of mature OLs. Together, these data revealed that EGF is an important mitogen to enhance oligodendroglial development.