Capillary gas chromatographic separation of urinary organic acids. Retention indices of 101 urinary acids on a 5% phenylmethyl silicone capillary column.
Capillary gas chromatographic separation of urinary organic acids. Retention indices of 101 urinary acids on a 5% phenylmethyl silicone capillary column.
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毛细管%20气体%20色谱%20分离%20of%20尿%20有机%20酸%20保留%20指数%20of%20101%20尿%20酸%20on%20a%205%%20苯甲基%20有机硅%20毛细管%20柱。
DOI：
10.1093/chromsci/22.5.198
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发表时间：
1984
影响因子：
1.3
通讯作者：
W. Krivit
中科院分区：
文献类型：
--
作者：
M. Tuchman;L. Bowers;K. Fregien;P. J. Crippin;W. Krivit
报道了 101 种尿液有机酸的三甲基硅基和肟化三甲基硅基衍生物在 5% 苯甲基硅酮熔融石英毛细管柱上的气相色谱保留指数（亚甲基单位）。使用阴离子交换色谱法，从五名健康个体、七名神经母细胞瘤患者和九名遗传性有机酸尿症患者的尿液中提取有机酸。通过毛细管气相色谱法实现各种酸的分离，并使用计算机库搜索程序通过质谱法进行鉴定。所有鉴定均通过与参考质谱的目视比较来确认。所有酸的保留指数标准偏差均小于 0.035 亚甲基单元，46 种酸的保留指数标准偏差小于 0.01 亚甲基单元。显示了三张来自神经母细胞瘤、苯丙酮尿症和丙酸血症患者的尿液色谱图以及一张来自健康个体的尿液色谱图。
Gas chromatographic retention indices (methylene units) are reported for 101 urinary organic acids as their trimethylsilyl and oximated trimethylsilyl derivatives on a 5% phenylmethyl silicone fused silica capillary column. Using anion exchange chromatography, organic acids were extracted from urines of five healthy individuals, seven patients with neuroblastoma, and nine patients with inherited organic acidurias. Separation of the various acids was achieved by capillary gas chromatography and identification was done by mass spectrometry using a computerized library search program. All identifications were confirmed by visual comparison with reference mass spectra. Standard deviations of the retention indices for all acids were less than 0.035 methylene units and for 46 acids less than 0.01 methylene units. Three chromatograms of urine from individuals with neuroblastoma, phenylketonuria, and propionic acidemia and one from a healthy individual are shown.