Identification of microRNA and bioinformatics target gene analysis in beef cattle intramuscular fat and subcutaneous fat.
Identification of microRNA and bioinformatics target gene analysis in beef cattle intramuscular fat and subcutaneous fat.
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DOI：
10.1039/c3mb70084d
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发表时间：
2013-07
影响因子：
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通讯作者：
Haiyang Wang;Yueying Zheng;Genlin Wang;Hui-Xia Li
中科院分区：
文献类型：
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作者：
Haiyang Wang;Yueying Zheng;Genlin Wang;Hui-Xia Li
microRNA（miRNA）是一种内源性非编码RNA，在体内脂肪形成过程中起重要作用。然而，miRNA的差异表达以及这些miRNA在牛肌内和皮下脂肪组织中的作用仍然未知。本研究利用微阵列技术和生物信息学技术，对成年肉牛肌内脂肪和皮下脂肪组织中差异表达的miRNA进行了研究，并对差异表达的目的基因进行了分析。在213个miRNAs中，共鉴定出88个差异表达的miRNAs，其中30个miRNAs在肌内脂肪和皮下脂肪中差异显著（倍数变化>1，P < 0.001）。miR-143、miR-145、miR-26 a、miR-2373- 5 p和miR-23 b-3 p在肌内脂肪中高度表达，而miR-26 a、miR-2373- 5 p、miR-2325 c、miR-3613和miR-2361在皮下脂肪中显示最高丰度。KEGG通路分析和GO术语富集的生物信息学提示，这些差异表达的miRNAs参与不同的通路和靶基因，可能对脂肪沉积有不同的调控作用。综上所述，本研究为更好地了解miRNA在牛脂肪沉积中的差异表达及其机制提供了第一手证据，并为通过减少肉牛皮下脂肪和增加肌内脂肪来改善牛肉品质提供了思路。
MicroRNA (miRNA) is endogenous non-coding RNA that has been proposed to play an important role in the formation of body fat. However, the differential expression of miRNA and the role of these miRNAs in bovine intramuscular and subcutaneous fat tissue are still unknown. In this study, the profile of differentially expressed miRNA and the target gene analysis in intramuscular adipose and subcutaneous adipose of adult beef cattle were investigated by microarray and bioinformatics. The data identified 88 differentially expressed miRNAs in 213 miRNAs which were detected on the microarray, and 30 miRNAs among these 88 miRNAs were changed significantly between intramuscular and subcutaneous fat (fold change >1, P < 0.001). miR-143, miR-145, miR-26a, miR-2373-5p and miR-23b-3p were highly expressed in intramuscular fat, whilst miR-26a, miR-2373-5p, miR-2325c, miR-3613 and miR-2361 showed highest abundance in subcutaneous fat. Bioinformatics of KEGG pathway analysis and GO term enrichment suggested that these differentially expressed miRNAs involved in different pathways and target genes may regulate differently the fat deposition. Taken together, our study provides the first evidence for better understanding the differential expression and mechanisms of miRNA in bovine fat deposition, and provides thinking to improve the quality of beef by reducing subcutaneous fat and increasing intramuscular fat of beef cattle.