miR-410-3p promotes prostate cancer progression via regulating PTEN/AKT/mTOR signaling pathway
miR-410-3p promotes prostate cancer progression via regulating PTEN/AKT/mTOR signaling pathway
复制标题
miR-410-3p 通过调节 PTEN/AKT/mTOR 信号通路促进前列腺癌进展。
DOI：
10.1016/j.bbrc.2018.06.176
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发表时间：
2018-09-18
影响因子：
3.1
通讯作者：
Zhang, Qi
中科院分区：
文献类型：
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作者：
Zhang, Yuelong;Zhang, Dahong;Zhang, Qi
前列腺癌已成为男性最常见的泌尿系统肿瘤之一。近年来，miRNAs因其在人类肿瘤中的特殊调控功能而不断受到研究者的关注。先前的研究表明，miR-410可作为前列腺癌诊断的生物标志物。然而，miR-410- 3 p在前列腺癌中的具体生物学功能尚不清楚。本研究旨在探讨miR-410- 3 p在前列腺癌中的作用及其分子机制。通过qRT-PCR检测miR-410- 3 p在前列腺癌组织和细胞系中的高表达。然后用Kaplan Meier法确定其预后价值。miR-410- 3 p高表达提示前列腺癌患者预后不良。为了研究miR-410- 3 p在前列腺癌中的生物学功能，设计并在两种前列腺癌细胞系（PC 3和DU 145）中进行功能丧失测定。因此，下调的miR-410- 3 p抑制细胞增殖、迁移和EMT进展。流式细胞仪检测miR-410- 3 p对细胞凋亡的促进作用。从机制上讲，进一步的分析表明，miR-410- 3 p的作用通过下调PTEN发挥致癌功能。本研究的所有结果表明，miR-410- 3 p通过下调PTEN/AKT/mTOR信号通路抑制前列腺癌进展。(C)2018爱思唯尔公司All rights reserved.
Prostate cancer has become one of commonest urologic tumors in male. In recent years, miRNAs are continually attracting attentions of researchers for their special regulatory function in human cancers. Previous study has revealed that miR-410 acts as a biomarker for the diagnosis of prostate cancer. Whereas, the specific biological function of miR-410-3p in prostate cancer remains unknown. The aim of this study is to explore the function and molecular mechanism of miR-410-3p in prostate cancer. The high expression of miR-410-3p was examined in prostate cancer tissues and cell lines by qRT-PCR. Next, the prognostic value was identified by Kaplan Meier method. High expression of miR-410-3p indicated poor prognosis of prostate cancer patients. To investigate the biological function of miR-410-3p in prostate cancer, loss-of function assays were designed and performed in two prostate cancer cell lines (PC3 and DU145). As a result, downregulated miR-410-3p suppressed cell proliferation, migration and EMT progress. Moreover, flow cytometry analysis was performed to determine that the acceleration effects of miR-410-3p on cell apoptosis. Mechanistically, further analysis demonstrated that the effects of miR-410-3p exert oncogenic functions through downregulating PTEN. All findings in this study revealed that miR-410-3p inhibits prostate cancer progression via downregulating PTEN/AKT/mTOR signaling pathway. (C) 2018 Elsevier Inc. All rights reserved.