Rice lesion mimic mutants with enhanced resistance to diseases
Rice lesion mimic mutants with enhanced resistance to diseases
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DOI：
10.1007/s00438-008-0337-2
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发表时间：
2008-03
影响因子：
3.1
通讯作者：
Changjian Wu;A. Bordeos;M. Madamba;M. Baraoidan;M. Ramos;Guo‐Liang Wang;J. Leach;H. Leung
中科院分区：
文献类型：
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作者：
Changjian Wu;A. Bordeos;M. Madamba;M. Baraoidan;M. Ramos;Guo‐Liang Wang;J. Leach;H. Leung
病变模拟突变体的特征在于在没有病原体的情况下形成坏死病变。这种遗传缺陷通常导致对病原体感染的抗性增强和防御反应基因的组成型表达。为了了解导致这些突变的遗传机制，我们对从IR 64水稻突变体群体中分离的21个病变模拟突变体进行了表征，这些突变体是通过二环氧丁烷（D）、γ射线（G）和快中子（F）诱变产生的。四个突变由单个显性基因控制，其中一个是母系遗传的。5个病变模拟突变体与已知的斑点叶（spl）突变体spl 1、spl 2、spl 3或spl 6是等位的。基于等位性测试，总共建立了11个新的病变模拟突变体，命名为spl 16、spl 17和spl 19至spl 27。两个病斑模拟物spl 17和Spl 26对稻瘟病病原菌Magnaporthe magnosa和白叶枯病病原菌Xanthomonasoryzaepv.共分离分析分离群体的spl 17和spl 26的稻瘟病和BB抗性和病变模拟表型表明，增强的抗性，这两种疾病是由病变模拟基因的突变。从两个独立的病变模拟产生的双突变体表现出更严重的病变和更高水平的抗X。稻瘟病菌的突变体比它们的单突变体亲本的突变体更显著，表明两种突变的协同效应。在对两种病原体表现出增强的抗病性的突变体中，防御反应基因PR-10a、POX22.3和PO-C1表达的增加与模拟病害的发展和抗性的增强相关。这些拟病斑突变体为全面研究水稻抗病途径提供了重要材料。
Lesion mimic mutants are characterized by the formation of necrotic lesions in the absence of pathogens. Such genetic defects often result in enhanced resistance to pathogen infection and constitutive expression of defense response genes. To understand the genetic mechanisms leading to these mutations, we characterized 21 lesion mimic mutants isolated from IR64 rice mutant populations produced by mutagenesis with diepoxybutane (D), gamma rays (G), and fast neutrons (F). Four mutations are controlled by single dominant genes, one of which is inherited maternally. Five lesion mimics are allelic to known spotted leaf (spl) mutantsspl1,spl2,spl3,or spl6.In total, 11 new lesion mimic mutations, named spl16, spl17, and spl19 through Spl27, were established based on allelism tests. Two lesion mimics,spl17 andSpl26 showed enhanced resistance to multiple strains ofMagnaporthe oryzae, the rice blast pathogen, andXanthomonas oryzaepv.oryzae, the bacterial blight (BB) pathogen. Co-segregation analyses of blast and BB resistance and lesion mimic phenotypes in segregating populations ofspl17 andSpl26 indicate that enhanced resistance to the two diseases is conferred by mutations in the lesion mimic genes. A double mutant produced from two independent lesion mimics showed more severe lesions and higher level of resistance toX. o.pv.oryzaethan their single mutant parents indicating a synergistic effect of the two mutations. In mutants that exhibit enhanced disease resistance to both pathogens, increases in expression of defense response genesPR-10a,POX22.3, andPO-C1were correlated with lesion mimic development and enhancement of resistance. These lesion mimic mutants may provide essential materials for a comprehensive dissection of the disease resistance pathways in rice.