ON-LINE MONITORING OF CELL SIZE DISTRIBUTION IN MAMMALIAN CELL CULTURE PROCESSES
ON-LINE MONITORING OF CELL SIZE DISTRIBUTION IN MAMMALIAN CELL CULTURE PROCESSES
复制标题
哺乳动物细胞培养过程中细胞大小分布的在线监测
DOI：
--
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
A. Kamen
中科院分区：
文献类型：
--
作者：
O. Henry;S. Ansorge;M. Aucoin;R. Voyer;A. Kamen
摘要在这项工作中，哺乳动物细胞培养在线监测通过测量在一个范围内的激励频率的培养电容。在补料分批培养过程中收集的数据用于建立偏最小二乘（PLS）模型，用于预测细胞浓度，活力和大小分布。这些模型在批量和灌注模式下进行的培养物上进行了验证，预测结果与库尔特计数器进行的离线测量结果非常一致。电容信号还可用于跟踪培养期间平均细胞直径的演变。细胞大小分布提供了有关培养物中细胞生理状态的有价值的信息。因此，在多个频率下的电容测量可以是用于细胞培养过程的开发、优化、监测和控制的有用工具。
Abstract In this work, mammalian cell cultures were monitored on-line by measuring the culture capacitance over a range of excitation frequencies. The data collected during a fed-batch culture were used to build partial least squares (PLS) models for predicting the cell concentration, viability and size distribution. The models were validated on cultures performed in batch and perfusion modes and the predictions agreed well with off-line measurements performed with a Coulter Counter. The capacitance signals can also be used to follow the evolution of the mean cell diameter during a culture. Cell size distribution provides valuable information about the physiological state of cells in culture. Capacitance measurements at multiple frequencies can thus be a useful tool for the development, optimization, monitoring and control of cell culture processes.