Functional Group Distribution of the Carrier Surface Influences Adhesion of Methanothermobacter thermautotrophicus
Functional Group Distribution of the Carrier Surface Influences Adhesion of Methanothermobacter thermautotrophicus
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DOI：
10.1155/2020/9432803
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发表时间：
2020-01
期刊：
影响因子：
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通讯作者：
Masaki Umetsu;Takaaki Sunouchi;Y. Fukuda;Hideyuki Takahashi;C. Tada
中科院分区：
文献类型：
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作者：
Masaki Umetsu;Takaaki Sunouchi;Y. Fukuda;Hideyuki Takahashi;C. Tada
在厌氧废水处理系统中，各种载体被用于高密度保留产甲烷古菌。尽管载体材料和微生物的物理化学性质影响产甲烷古菌的黏附，但关于其潜在机制的细节仍未得到很好的描述。我们对碳毡进行了七种化学表面修饰，以阐明具有代表性的嗜热嗜氢产甲烷菌--甲烷热营养杆菌的粘附性。考察了载体表面性质与产甲烷菌粘附性的关系。在NaOH、HCl、H_2SO_4或Na_2HPO_4处理的碳毡上，热营养支原体的粘附率显著增加，最高可达对照的2.6倍。经处理的炭毡具有较低的水接触角，但载体表面接触角与甲烷附着力之间没有相关性。而在甲烷菌附着力增强的载体表面，-COOH：-OH的比例为1：0.65。在处理过的载体中没有观察到这样的比率，因为它没有改善甲烷菌的粘附性。因此，在嗜热中营养分枝杆菌的黏附过程中，官能团的丰度以及疏水性等物理表面性质都是重要的。在厌氧消化启动过程中，产氢产甲烷菌参与了活性甲烷化反应。此外，这些产甲烷古菌还起到产甲烷阴极催化剂的作用。因此，通过控制嗜热嗜酸杆菌等产氢产甲烷菌的粘附性，将大大提高厌氧消化性能。
Various support carriers are used for high-density retention of methanogenic archaea in anaerobic wastewater treatment systems. Although the physicochemical properties of carrier materials and microorganisms influence the adhesion of methanogenic archaea, details about the underlying mechanism remain poorly characterized. We applied seven types of chemical surface modifications to carbon felts to clarify the adhesion properties of Methanothermobacter thermautotrophicus, a representative thermophilic hydrogenotrophic methanogen. The relationship between carrier surface properties and methanogen adhesion was evaluated. M. thermautotrophicus adhesion was significantly increased up to 2.6 times in comparison with control on carbon felts treated with NaOH, HCl, H2SO4, or Na2HPO4. Treated carbon felts showed a lower water contact angle, but no correlation between the carrier surface contact angle and methanogen adhesion was observed. On the other hand, at the surface of the carrier that showed improved adhesion of methanogens, the ratio of -COOH : -OH was 1 : 0.65. Such a ratio was not observed with treated carriers for which methanogen adhesion was not improved. Therefore, in the adhesion of M. thermautotrophicus, the functional group abundance was important as well as physical surface properties such as the hydrophobicity. Hydrogenotrophic methanogens are involved in active methanation during the startup of anaerobic digestion. Additionally, these methanogenic archaea function as methanogenic cathode catalysts. Therefore, anaerobic digestion performance will greatly improve by controlling the adhesion of hydrogenotrophic methanogens such as M. thermautotrophicus.