Acute exposure to environmentally relevant concentrations of Chinese PFOS alternative F-53B induces oxidative stress in early developing zebrafish.
Acute exposure to environmentally relevant concentrations of Chinese PFOS alternative F-53B induces oxidative stress in early developing zebrafish.
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急性暴露于环境相关浓度的中国 PFOS 替代品 F-53B 会在早期发育的斑马鱼中诱导氧化应激。
DOI：
10.1016/j.chemosphere.2019.07.016
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Tu Wenqing
中科院分区：
文献类型：
--
作者：
Wu Yongming;Huang Jing;Deng Mi;Jin Yuanxiang;Yang Huilin;Liu Yu;Cao Qinyan;Mennigen Jan A;Tu Wenqing
6：2氯化多氟醚磺酸盐（F-53 B）是中国的一种全氟辛烷磺酸替代品，最近在水生环境中发现其浓度类似于或高于全氟辛烷磺酸。尽管先前的研究表明F-53 B可以引发鱼类的氧化应激，但其潜在的分子机制仍然很大程度上未知。本研究以斑马鱼胚胎为材料，研究了不同浓度F-53 B（0、0. 5、20和200 μg/L）对斑马鱼胚胎氧化应激反应的影响及其可能的分子机制。我们的研究结果表明，F-53 B在斑马鱼幼虫中以浓度依赖性方式积累。丙二醛（MDA）和还原型谷胱甘肽（GSH）含量以及参与磷脂酰肌醇3-激酶（PI 3 K）/Akt/Nrf 2-ARE途径的大部分抗氧化酶基因的活性、mRNA和蛋白水平均显著降低。进一步的计算机模拟研究表明，F-53 B与PI 3 K紧密结合，这可能与F-53 B作为PI 3 K抑制剂抑制Nrf 2调节的抗氧化功能有关。结合体内和计算机模拟研究，阐明了F-53 B通过PI 3 K/Akt/Nrf 2-ARE途径对斑马鱼抗氧化系统的影响，加深了我们对F-53 B影响鱼类抗氧化反应的分子机制的理解。
6:2 chlorinated polyfluorinated ether sulfonate (F-53B), a Chinese PFOS alternative, has recently been identified in the aquatic environment at concentrations similar to or higher than perfluorooctane sulfonate (PFOS). Although previous studies have shown that F-53B can trigger oxidative stress in fish, the underlying molecular mechanism is still largely unknown. In this study, zebrafish embryos were exposed to various concentrations of F-53B (0, 0.5, 20 and 200 μg/L) for 5 d to investigate oxidative stress responses and possible molecular mechanisms of action. Our results showed that F-53B accumulated in a concentration-dependent manner in zebrafish larvae. The contents of malondialdehyde (MDA) and reduced glutathione (GSH), as well as the activities, mRNA and protein levels of most of antioxidant enzyme genes involved in the phosphatidylinositol 3-kinase (PI3K)/Akt/Nrf2-ARE pathway were significantly reduced. Furtherin silicostudy indicated that F-53B binds tightly to PI3K, which may be related to the inhibition of Nrf2-regulated antioxidant functions by F-53B as a PI3K inhibitor. Combiningin vivoandin silicostudies, we elucidated the effects of F-53B on antioxidant system of zebrafish through the PI3K/Akt/Nrf2-ARE pathway, which increases our understanding of the molecular mechanism of F-53B on antioxidant responses in fish.